3300027458: Soil microbial communities from Arlington Agricultural Research Station in Wisconsin, USA - Nitrogen cycling UWRJ-ROWE17-B (SPAdes)

Overview

Basic Information

• IMG/M Taxon OID: 3300027458
• GOLD Reference (Study | Sequencing Project | Analysis Project): Gs0095510 | Gp0072141 | Ga0207602
• Sample Name: Soil microbial communities from Arlington Agricultural Research Station in Wisconsin, USA - Nitrogen cycling UWRJ-ROWE17-B (SPAdes)
• Sequencing Status: Permanent Draft
• Sequencing Center: DOE Joint Genome Institute (JGI)
• Published?: N
• Use Policy: Open

Dataset Contents

• Total Genome Size: 13715273
• Sequencing Scaffolds: 38
• Novel Protein Genes: 43
• Associated Families: 41

Dataset Phylogeny
Taxonomy Groups	Number of Scaffolds
All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	14
All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	7
All Organisms → cellular organisms → Bacteria → Terrabacteria group → Chloroflexi → unclassified Chloroflexi → Chloroflexi bacterium	1
All Organisms → cellular organisms → Bacteria → Terrabacteria group	1
All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia	3
All Organisms → cellular organisms → Bacteria	3
All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Acidimicrobiia → unclassified Acidimicrobiia → Acidimicrobiia bacterium	1
All Organisms → cellular organisms → Bacteria → Proteobacteria → Alphaproteobacteria	1
All Organisms → cellular organisms → Bacteria → Acidobacteria	2
All Organisms → cellular organisms → Bacteria → Acidobacteria → unclassified Acidobacteria → Acidobacteria bacterium	2
Not Available	2
All Organisms → cellular organisms → Bacteria → Acidobacteria → Blastocatellia → unclassified Blastocatellia → Blastocatellia bacterium	1

Ecosystem and Geography

Ecosystem Assignment (GOLD)

• Name: Soil Microbial Communities From Arlington Agricultural Research Station In Wisconsin And Kellogg Biological Station In Michigan, Replicating The Bioenergy Cropping Systems Trials (Bcsts)
• Type: Environmental
• Taxonomy: Environmental → Terrestrial → Soil → Unclassified → Agricultural Land → Soil → Soil Microbial Communities From Arlington Agricultural Research Station In Wisconsin And Kellogg Biological Station In Michigan, Replicating The Bioenergy Cropping Systems Trials (Bcsts)

Alternative Ecosystem Assignments

• Environment Ontology (ENVO): terrestrial biome → agricultural field → agricultural soil
• Earth Microbiome Project Ontology (EMPO): Free-living → Non-saline → Soil (non-saline)

Location Information

• Location: Wisconsin, United States
• Coordinates
  • Lat. (^o): 43.42
  • Long. (^o): -89.32
  • Alt. (m): N/A
  • Depth (m): 0

Associated Families

Family	Category	Number of Sequences	3D Structure?
F000268	Metagenome / Metatranscriptome	1411	Y
F001033	Metagenome / Metatranscriptome	799	Y
F003216	Metagenome / Metatranscriptome	500	Y
F004245	Metagenome	447	Y
F005987	Metagenome / Metatranscriptome	384	Y
F006680	Metagenome / Metatranscriptome	367	Y
F007494	Metagenome / Metatranscriptome	350	Y
F008399	Metagenome / Metatranscriptome	334	Y
F009768	Metagenome / Metatranscriptome	313	Y
F009821	Metagenome / Metatranscriptome	312	Y
F009996	Metagenome / Metatranscriptome	310	Y
F013034	Metagenome / Metatranscriptome	275	Y
F013923	Metagenome / Metatranscriptome	267	Y
F015237	Metagenome	256	Y
F015540	Metagenome / Metatranscriptome	254	N
F020731	Metagenome / Metatranscriptome	222	Y
F020901	Metagenome / Metatranscriptome	221	Y
F030191	Metagenome / Metatranscriptome	186	N
F030421	Metagenome / Metatranscriptome	185	Y
F030533	Metagenome / Metatranscriptome	185	N
F030749	Metagenome / Metatranscriptome	184	N
F031666	Metagenome / Metatranscriptome	182	Y
F032746	Metagenome / Metatranscriptome	179	Y
F033177	Metagenome / Metatranscriptome	178	Y
F037244	Metagenome	168	Y
F048295	Metagenome / Metatranscriptome	148	Y
F049607	Metagenome / Metatranscriptome	146	Y
F051582	Metagenome / Metatranscriptome	144	Y
F053350	Metagenome / Metatranscriptome	141	Y
F053485	Metagenome / Metatranscriptome	141	Y
F055393	Metagenome / Metatranscriptome	138	N
F056809	Metagenome / Metatranscriptome	137	N
F057238	Metagenome / Metatranscriptome	136	N
F058987	Metagenome / Metatranscriptome	134	N
F063961	Metagenome / Metatranscriptome	129	Y
F068182	Metagenome / Metatranscriptome	125	Y
F076437	Metagenome	118	Y
F084685	Metagenome / Metatranscriptome	112	N
F089237	Metagenome / Metatranscriptome	109	Y
F098110	Metagenome	104	N
F101117	Metagenome / Metatranscriptome	102	N

Associated Scaffolds

Scaffold	Taxonomy	Length
Ga0207602_100268	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	1288
Ga0207602_100292	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	1258
Ga0207602_100487	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	1094
Ga0207602_100611	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Chloroflexi → unclassified Chloroflexi → Chloroflexi bacterium	1029
Ga0207602_100938	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	921
Ga0207602_100995	All Organisms → cellular organisms → Bacteria → Terrabacteria group	904
Ga0207602_101082	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	875
Ga0207602_101479	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	793
Ga0207602_101481	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	792
Ga0207602_101508	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia	788
Ga0207602_101666	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	763
Ga0207602_101723	All Organisms → cellular organisms → Bacteria	755
Ga0207602_101732	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	753
Ga0207602_101813	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	742
Ga0207602_101887	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	732
Ga0207602_101935	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Acidimicrobiia → unclassified Acidimicrobiia → Acidimicrobiia bacterium	725
Ga0207602_101960	All Organisms → cellular organisms → Bacteria → Proteobacteria → Alphaproteobacteria	723
Ga0207602_102367	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	679
Ga0207602_102475	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	668
Ga0207602_102608	All Organisms → cellular organisms → Bacteria → Acidobacteria	656
Ga0207602_103521	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	590
Ga0207602_103528	All Organisms → cellular organisms → Bacteria → Acidobacteria	590
Ga0207602_103730	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	579
Ga0207602_103838	All Organisms → cellular organisms → Bacteria → Acidobacteria → unclassified Acidobacteria → Acidobacteria bacterium	573
Ga0207602_103885	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	570
Ga0207602_104039	All Organisms → cellular organisms → Bacteria	563
Ga0207602_104403	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	548
Ga0207602_104661	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia	537
Ga0207602_104690	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia → unclassified Verrucomicrobia → Verrucomicrobia bacterium	536
Ga0207602_104794	All Organisms → cellular organisms → Bacteria → Acidobacteria → unclassified Acidobacteria → Acidobacteria bacterium	532
Ga0207602_104834	All Organisms → cellular organisms → Bacteria	530
Ga0207602_104876	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	529
Ga0207602_104916	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	527
Ga0207602_105295	Not Available	514
Ga0207602_105374	All Organisms → cellular organisms → Bacteria → PVC group → Verrucomicrobia	511
Ga0207602_105378	All Organisms → cellular organisms → Bacteria → Acidobacteria → Blastocatellia → unclassified Blastocatellia → Blastocatellia bacterium	511
Ga0207602_105640	Not Available	502
Ga0207602_105661	All Organisms → cellular organisms → Bacteria → Terrabacteria group → Actinobacteria → Actinomycetia → unclassified Actinomycetia → Actinomycetia bacterium	501

Sequences

Scaffold ID	Protein ID	Family	Sequence
Ga0207602_100268	Ga0207602_1002681	F008399	MVRSLRSFRSVRGAWRFPIVVAVGVIVASSCSSSSSSGGGGTSLAATIWVQNFKYNDVPASFPSGVVELLFQNRESFPITHEMIPIALPEGKTAQDVIDAAKAPGDAGPASEDEWLHIGGDFGAVDTGAGIVEILNLPPGNYAFACWQTG
Ga0207602_100292	Ga0207602_1002921	F006680	MKISKFLWSVLVAFAAVALIAPQQVEAIVVDGRIKGQIQFTGSGTVTESTGINSNAINSNGINELEFNGENFPHGPLSVTGGTGDFIPFVGSQATFNLAIRWTGSGSSVNLLDVLPGVGGPAWNIFITGDGNATGTLFS
Ga0207602_100487	Ga0207602_1004871	F084685	MSDGGRERASLGVEVWNSSQKWSVQRSAVRSIAWLDVLCAVKSRPNILRPLERFSLFRDVLPERVGQIGRDVVPGFGGESIEKGPVFITDPAPREPPDRAIGRVAEQAPQDGTVGRPALGIQHAQMKLKEAVFVAPEHRGHVTVQDEAILIRQSFAVGGARYDVANPGIAQAREEFDVARASSLLH
Ga0207602_100611	Ga0207602_1006112	F051582	MPKRIALKDSVEVDSVDLSDFARSVRFTSEHEQVDVSGFNATGANEYLAGTTTQNVTVEFFGSYGTGEVHQTLYPIHQNRDVVAFQWRPDQTQPIAVDNPELRGNVQLLSYGPGAARGEVDTFEATFITADAAGLVFETTPLP
Ga0207602_100938	Ga0207602_1009383	F053350	MLELLDLRERGGRLEPSKLEIDPATTEIVRQILQRVFVEGDDVLIELSKLFDGADLSRSGILVTEREFAHAERETP
Ga0207602_100981	Ga0207602_1009812	F068182	GAFWLSIDGTRGGFVVAPQAIWHGSEMARSFRGASSRGWHAEDVYRYWQRQVGAAGMFMIDPQQHADTLFQVARRVGAL
Ga0207602_100995	Ga0207602_1009952	F048295	MATVGERIEITAAQARAFLGSPTGVLFRRYLAAGVIVTAPLLFRIPGLRRYPLIRVLELVGGAALLIKAAEAIRDWDASGGNEQRIVIDV
Ga0207602_101082	Ga0207602_1010821	F005987	VLTLPERQDALGGWHQVLREVCYGCGCHYVSAEDDHDLVWEPGREVQRGCTDELCECHTAPVIGERRD
Ga0207602_101385	Ga0207602_1013851	F030191	WSRDGVAEKMDPVIEPTTVWMVHLDRTPTDETEGILSADEWELVFVDAGSPETTRFPFVDIVNVKRVLGSPVFTLGWRFHDERRQTAFYLTRPPPLGTLPPGGGPPDIPDLRAATTLRRSGRWRQRRDNTRYLVATSTSLKEQRDTLVSQIKAAMKQARGEPS
Ga0207602_101479	Ga0207602_1014792	F031666	MKLRTLIMFGAGIMTGLAIARKMSEDDPEVQHGPTRSYESNPAVRAVTSQAQRVADRATVASLAAIRRARGALRERLAEEQYDDVNWN
Ga0207602_101481	Ga0207602_1014812	F001033	FVSRTKSMAVPHQILGASTNEKRELLVCGHSLIARLTIAVFVFQMLGVTSVVHAERPDSTAGTSSAGTRKLFIGPSSASVALGKASLIVSPLTHRDGNYVGDYQLKVRPYFFKSEKGSLLLAASDDAVRKLQAGTAINFTGQAVTHKDGRTHIVLGRATPSSSDRGSVTFSIVTDDARIVFNTSYHFPAPPP
Ga0207602_101508	Ga0207602_1015081	F004245	SNLSNSDRSADVRDDWGWRSDVSVNNGLQLTRDLRLNLGADVRGDVWDRFDAFNTVGVGASAGLRYRFGLGRQAPWILLEDRFGYDRFHDTPQSGNDNVVNLRGGVALTDRIALEGGYAFESFVAPNDFYDRQLHRADVRMVFDVTSSLQLGLGYSYQEGDVISYAVPPRPDIARFSVEREDQDEFGQPLRTAYKLLGRTHALSFSAAYQLTKRASVQLGYEYAITTHDPLEYENHLVEVKIAVAY
Ga0207602_101666	Ga0207602_1016662	F030421	MDPVRTLDTGIASGLVTVLVDELGGGSSTIQTPAADFEAAERGVAIGRRFIPWHRVRRYEWDLPPKEFGEEHRVSASVRLVVDDANGVPEEHVVSADGFEASAFAVTMLLEDAAGGGTIRVRRVGIPWHHVREYERIPADATLDGDTPERPDA
Ga0207602_101723	Ga0207602_1017231	F032746	TPENSGDWPNGKSYPVTAAQLHRLHTGFLAPIHCFKLAKN
Ga0207602_101732	Ga0207602_1017322	F056809	MKIRNNSQSRAASALVAVIITITILALLASSLVINVTNRRMTVSQASAWQEALAAAEAGVHQGIAQLEQGLAQNSLPLAGPSPIAWSSPFPSGTATSTSTRVYLDHAGEGPSGPSNSYADYTLTLNENTTQTIPRPYYCIVSVGTVGLPGGKALGMDSADAVLRKLNLQTATRTATRTIEAW
Ga0207602_101813	Ga0207602_1018132	F020901	RAVKVLCVATDEDALARLRRASVGAEWELVAGATDLRGALDQLDVERPHALVVFGPFAELVALAAERFPGMRIITDRDAPGASAVATSMEDVRGLLAEQPRPGGPVR
Ga0207602_101887	Ga0207602_1018872	F033177	MSGDDDLRKLLATLDPQARNDLRRVLIHDQADRDAIASQLLRYRDEHGDDWTDIIDMLTMHPEVRRLLVRVLGELEADHRTG
Ga0207602_101935	Ga0207602_1019351	F009821	MGEPESTVCPICGKGTLITIDFGEQQPESRQVQTFTCGHEVEGARLQTANAEELDVERRTSEETAQPTDPEE
Ga0207602_101960	Ga0207602_1019601	F063961	HGLTGPRNATAILRMKQHATCTQKIKSLGIAPLVERPVRTLNQSTLCLDDQSERGHATTADAAKKVISKLGHWPNLEGLPMGCNAGWAIG
Ga0207602_102367	Ga0207602_1023672	F015237	MMKTFLLLAVGSVITAGALPSLPAKIILSAFVLMAVLTQAARRAPAGYEDENGFHLIRPRRPVAEGR
Ga0207602_102475	Ga0207602_1024751	F058987	LEAWEGRRLQLDGQEFLRFRGLWQREDVPALVEEWGCLGIDRAAARSEARSKQGWTQLLVDHPARPGAMYSELFADWIRLSGLMDYAVGLLGLLQDHAAVGDLVDALRGPLGSRPRDPEQIRADLPDEGWLRGILGGAARMNMSCEFEADTNSMTLVSRDP
Ga0207602_102608	Ga0207602_1026081	F007494	MKQNLKVSLGAVVLALATLAAMIFAWLNFVQRTEFESPDDGV
Ga0207602_102781	Ga0207602_1027812	F033177	VVRMTEGLRSLLETLDPKARDDLRRVLIHDQPDRDAIALQRMRYRDENGQRWADIIDALTMNPDARRQVARLLGEIDASK
Ga0207602_103521	Ga0207602_1035211	F009996	QTVISTILTIFVFFIVGRGDADPYKAFYVLYAGVTIVWCISTALLFLDIFFAKRAEPERFERERRISVGWLYVCGAVGFVVNILAVGFIFVGSWYPTGFPDLKVWNYWMVAFTAVSVITGVAIYLISQQTRRGKTDVELIGEGAATQEIAGDTSLA
Ga0207602_103528	Ga0207602_1035282	F030533	PPAPVEDIPEAAELTAEEAAALDFLQKDTEADAATEG
Ga0207602_103730	Ga0207602_1037302	F033177	MQEQDAALRALLESLDPLARDALRRVLIHDQADRDAVASQLLRYRDGRGDDWADIIDMLTMHPEARRKIVRLLGETQAESR
Ga0207602_103838	Ga0207602_1038381	F003216	MTLKNAALLALIGTILATVLLVWTFVMTVLNVQRGLAPPLMLFPWFIYAFAGFSVAVFFYVFQKAQS
Ga0207602_103885	Ga0207602_1038852	F009768	LFLQIATEFATIGSMFSPEQSIRPGAEDKLAALRRLDKSRAWNCLDDRLYCTICKREISGRQIEVVGGTNGLRALHLKCSTPGCFSTPADWILPVKSDTVREIDIRFRDNGA
Ga0207602_104039	Ga0207602_1040391	F053485	LEELEIGPHAIQLHREIFGLHLDFENLPQIANGLVPAERQKRDFLPGIISRGKKRKALDVVPVKVREDDKNLFLLVTDGTEVSAQISQSRACVKDGDAVRIGERDLQAGGVSAEFLKTGIADGDGSACTIKLELHTIVFIKVSPRLTSSKQRIF
Ga0207602_104403	Ga0207602_1044031	F049607	QTMDAHPAAFVVVLTWARGEGLPVSYYAVTRDGRPITTLKPDTTTWIDRSVTPETRYVYVVAAVGADGTRALTHVVAKTLTAPLSTARFQGTFNVHLHAMSHFGFSDFHGENQTAGWRSTPACRKGPCDTELADLHRKVFVVTMTRNGAAYQGTVSISGIVKCAGADVTSTFTVSVHATDAG
Ga0207602_104548	Ga0207602_1045482	F015540	MPMPTVAIDVPSAQVLADQASIISELQFVMDCCKRLLADLAEPEEERDQLMPLALWSSAVLAYGRCFSKGKRFALSNDDVRNLPLHGAVMKFHQWILEERDKLAARAANPAEGAKVGAALSPPEQKDRRVEG
Ga0207602_104661	Ga0207602_1046612	F076437	ADQPVTGQEFKQRQIAFLNRIRDADPQQKVIDRAMLNERNELGLILDRNVEMNRIPDLMRTFLTQMASEFPGQDLTVIAYTPSNPPLKVGTAHLDSRTKEMNYLPEY
Ga0207602_104690	Ga0207602_1046901	F013034	MKSTCGRQWLFVATALALSGVWVTADDANSGEPLVQARFDQLIPTSYPTLRPSFDRLIQTTGPLTDPGIARVPITENLVRPLDQQLLIVNEGWGQLPAIRPQRESIPGLRQTSASRHAP
Ga0207602_104794	Ga0207602_1047941	F013923	MSRLDSKQRWSLVIGGILLTLLFAAVFTFGSLEVPFEPKSWRAMMPLFAVSCFMTAALIV
Ga0207602_104834	Ga0207602_1048341	F000268	MRVVAVMLLLSAGIAAEAMSYSFVSKASGRLGGPIRFEFYRDSTTRPKTDIESFTVSMRTADDRWKAMWSILSGRGLTQPIEYGVTPPGFTTMIQPQKLIPGRVYAGFATDGHGGSSGVTFGFDKDGTMIFPDSFDR
Ga0207602_104876	Ga0207602_1048761	F101117	MSMFNTNEPRTPGGRMGDDMEKMFLDEAKQDHLAKEAERADADAEHERAELGESVTTKRPWWKFWG
Ga0207602_104876	Ga0207602_1048762	F057238	GTKGRRMSTYERWRDVAGYRPGRHDNSGLRARLSWTKIEDRPIVSELRLAMQEREALTLPRHASSRR
Ga0207602_104916	Ga0207602_1049161	F030749	ELVPSFRAFHDVLDEIEPAKAGLTEVVPGTRLPGRPLDDALAEFLARLTRARGLMSAWRRPELEEEWSACTHGLVLALDRATQLLNDAYEATGFGSLVGLVEDLLDPLEPFAQAEDRFASLRRRTERPGQRARRGA
Ga0207602_105295	Ga0207602_1052951	F089237	MKVKIAMTFIALFIFAGATALATAQDTTKTTHKKTRTLTGCLQKGDSSNEYQLTTAKGGTWEIKSDTVKLGNHVGHTVTITGVLPNATLHGVKED
Ga0207602_105374	Ga0207602_1053741	F098110	LIKHQINEYAGDRDVKPDRHRPFCDPAMSVPAALEDRNESENDERERDKSEQNVARQHWKINCCQPTAKAGRFFPDVNVIGDIANEKKGGGNDRCNHANDVALPKISPDKIPAGRNENGTDKIERGIDCR
Ga0207602_105378	Ga0207602_1053782	F037244	MAHECVILADWPGALIELCGISTLERLLRTLQRCGIERATILSSTSERIAD
Ga0207602_105640	Ga0207602_1056402	F020731	IALLFVADAALEKDGSPVIVTSQRSGLPESSHRPDKIPVLTMAPAPDPDMTSKIVRDAQPKPVAQDPMKIHPAARAARAEAMPQTPSVTQPMNDRPPMNYHYRRSQEFDRFSIKGL
Ga0207602_105661	Ga0207602_1056611	F055393	MRRDPEGDVDESLEAREARLSEWADRKGYTLEKHGAGSPITTFDYSLVDKETFSIVIDSHDGLDRVEVFL