3300026422: Metatranscriptome of freshwater microbial communities from Mississippi River, Louisiana, United States - Miss_Colum_RepA_8d (Metagenome Metatranscriptome)

Overview

Basic Information

• IMG/M Taxon OID: 3300026422
• GOLD Reference (Study | Sequencing Project | Analysis Project): Gs0133460 | Gp0293501 | Ga0255308
• Sample Name: Metatranscriptome of freshwater microbial communities from Mississippi River, Louisiana, United States - Miss_Colum_RepA_8d (Metagenome Metatranscriptome)
• Sequencing Status: Permanent Draft
• Sequencing Center: DOE Joint Genome Institute (JGI)
• Published?: N
• Use Policy: Open

Dataset Contents

• Total Genome Size: 79628110
• Sequencing Scaffolds: 37
• Novel Protein Genes: 39
• Associated Families: 31

Dataset Phylogeny
Taxonomy Groups	Number of Scaffolds
All Organisms → cellular organisms → Eukaryota → Haptista → Haptophyta → Prymnesiophyceae → Coccolithales → Coccolithaceae → Coccolithus → Coccolithus braarudii	1
Not Available	24
All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage	2
All Organisms → cellular organisms → Eukaryota → Cryptophyceae	5
All Organisms → cellular organisms → Bacteria → Proteobacteria → Betaproteobacteria → Burkholderiales	1
All Organisms → cellular organisms → Eukaryota → Sar → Alveolata → Perkinsozoa → Perkinsea → Perkinsida → Perkinsidae → Perkinsus → unclassified Perkinsus → Perkinsus sp. BL_2016	2
All Organisms → cellular organisms → Bacteria	1
All Organisms → Viruses	1

Ecosystem and Geography

Ecosystem Assignment (GOLD)

• Name: Freshwater Microbial Communities Amended With Dissolved Organic Matter (Dom) From Various Rivers In The United States
• Type: Environmental
• Taxonomy: Environmental → Aquatic → Freshwater → River → Unclassified → Freshwater → Freshwater Microbial Communities Amended With Dissolved Organic Matter (Dom) From Various Rivers In The United States

Alternative Ecosystem Assignments

• Environment Ontology (ENVO): freshwater river biome → river → river water
• Earth Microbiome Project Ontology (EMPO): Free-living → Non-saline → Water (non-saline)

Location Information

• Location: USA: Louisiana
• Coordinates
  • Lat. (^o): 29.8571
  • Long. (^o): -89.9778
  • Alt. (m): N/A
  • Depth (m): 5

Associated Families

Family	Category	Number of Sequences	3D Structure?
F001904	Metagenome / Metatranscriptome	619	Y
F007472	Metagenome / Metatranscriptome	350	N
F016245	Metagenome / Metatranscriptome	248	N
F020340	Metagenome / Metatranscriptome	224	Y
F023359	Metagenome / Metatranscriptome	210	N
F025755	Metagenome / Metatranscriptome	200	Y
F026478	Metagenome / Metatranscriptome	197	Y
F027123	Metagenome / Metatranscriptome	195	Y
F032540	Metagenome / Metatranscriptome	179	N
F042749	Metagenome / Metatranscriptome	157	N
F044315	Metagenome / Metatranscriptome	154	N
F046147	Metagenome / Metatranscriptome	151	Y
F051067	Metagenome / Metatranscriptome	144	N
F051950	Metagenome / Metatranscriptome	143	Y
F059663	Metatranscriptome	133	N
F061852	Metagenome / Metatranscriptome	131	Y
F063745	Metagenome / Metatranscriptome	129	Y
F064751	Metagenome / Metatranscriptome	128	Y
F068878	Metagenome / Metatranscriptome	124	Y
F070194	Metagenome / Metatranscriptome	123	N
F070881	Metagenome / Metatranscriptome	122	Y
F070907	Metagenome / Metatranscriptome	122	Y
F072383	Metagenome / Metatranscriptome	121	Y
F079635	Metagenome / Metatranscriptome	115	N
F080126	Metagenome / Metatranscriptome	115	Y
F082176	Metagenome / Metatranscriptome	113	N
F086665	Metagenome / Metatranscriptome	110	N
F090468	Metagenome / Metatranscriptome	108	Y
F101271	Metagenome / Metatranscriptome	102	N
F103121	Metagenome / Metatranscriptome	101	Y
F104576	Metagenome / Metatranscriptome	100	Y

Associated Scaffolds

Scaffold	Taxonomy	Length
Ga0255308_1008289	All Organisms → cellular organisms → Eukaryota → Haptista → Haptophyta → Prymnesiophyceae → Coccolithales → Coccolithaceae → Coccolithus → Coccolithus braarudii	1265
Ga0255308_1011152	Not Available	1095
Ga0255308_1011373	Not Available	1084
Ga0255308_1015099	Not Available	939
Ga0255308_1016056	Not Available	909
Ga0255308_1016445	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage	897
Ga0255308_1016481	All Organisms → cellular organisms → Eukaryota → Cryptophyceae	896
Ga0255308_1016780	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage	888
Ga0255308_1017097	Not Available	880
Ga0255308_1017193	Not Available	877
Ga0255308_1018977	All Organisms → cellular organisms → Bacteria → Proteobacteria → Betaproteobacteria → Burkholderiales	835
Ga0255308_1019332	Not Available	827
Ga0255308_1023479	Not Available	747
Ga0255308_1025302	Not Available	718
Ga0255308_1026599	All Organisms → cellular organisms → Eukaryota → Sar → Alveolata → Perkinsozoa → Perkinsea → Perkinsida → Perkinsidae → Perkinsus → unclassified Perkinsus → Perkinsus sp. BL_2016	700
Ga0255308_1027135	Not Available	693
Ga0255308_1027323	All Organisms → cellular organisms → Eukaryota → Cryptophyceae	690
Ga0255308_1027499	Not Available	688
Ga0255308_1028532	All Organisms → cellular organisms → Eukaryota → Cryptophyceae	674
Ga0255308_1030983	Not Available	646
Ga0255308_1033591	All Organisms → cellular organisms → Eukaryota → Cryptophyceae	618
Ga0255308_1034640	All Organisms → cellular organisms → Bacteria	609
Ga0255308_1035097	All Organisms → Viruses	604
Ga0255308_1035108	All Organisms → cellular organisms → Eukaryota → Sar → Alveolata → Perkinsozoa → Perkinsea → Perkinsida → Perkinsidae → Perkinsus → unclassified Perkinsus → Perkinsus sp. BL_2016	604
Ga0255308_1041837	Not Available	552
Ga0255308_1042429	All Organisms → cellular organisms → Eukaryota → Cryptophyceae	549
Ga0255308_1043067	Not Available	544
Ga0255308_1043421	Not Available	542
Ga0255308_1043724	Not Available	540
Ga0255308_1043895	Not Available	539
Ga0255308_1044200	Not Available	537
Ga0255308_1044649	Not Available	535
Ga0255308_1046201	Not Available	525
Ga0255308_1046206	Not Available	525
Ga0255308_1046549	Not Available	523
Ga0255308_1048942	Not Available	510
Ga0255308_1048961	Not Available	510

Sequences

Scaffold ID	Protein ID	Family	Sequence
Ga0255308_1008289	Ga0255308_10082891	F001904	MKAVFLAVLALLALSAVAVSAQTDFEIAALEVAGDCHPECRWQCDDPVCPAQCHPVCERPKCQIHCEETECAKCKIHCDKPQCNVRCPKDLCEKNDCPKCETVCAPANCRTQCEAPNAVCTPMCEATKCDWKCKKPVTCPKPKCELVCERPKCAAKKRAAGPSFIETASTDKAENPTCCPCSVQANVAAAMQQASKNSEVDEEMMPSFMEVVHSMKFKEAASAENQCCPCAA
Ga0255308_1011152	Ga0255308_10111522	F072383	MKVTITYHDNQSFTVEEVVKLATDNYGRTAQVEVMPESTMAYDHIYFGLQQLVTHEQLSLLFERDASYQQDIKKLREEV
Ga0255308_1011373	Ga0255308_10113731	F079635	ASMAGTDPYYIDTDPEEAEGVCRPGYVRVMCWQNSACLQIREDVINQSQLMNFLASARPAENLSECIHLQNQLATPDNVKIICDWLAKHVEFQSRDAPAEERRAFDQRFFLLNPAGHAAYDAAANDQAKADILDSRKDWLRWRVDDPENEDPKRASVAPGVRTPEFKRTGPVEPRLHRTYEEIQHIMFVANFLELHPTDWHVDFAKKEAERIAAQPPGPFQEYGLDPADKFAETVMELCCHHLGDMILGCESPVDMQHRFPTLIGAKALTAKDRYDIVTADLWIHGDDFVEKVVPELQEQQRAEFAQARASGMSEDDIEKKFGRPIPDWTLESTQEERAFMKEVPGQEGNPTLRVPAIKI
Ga0255308_1015099	Ga0255308_10150991	F007472	MVYFTYKKLNICTVALLATVFAVLIAAMAVDWFSYKVEFSYTRVTATESSLSSSLYNYTQTTFDMFGQTVNVQRANTKIVQTSQQTYAQLGANGVNQQFKIQQAFVLIALLTAGLLFVAHTLYFFDGFRNKILFFIGITALRTILVIAFLAVVVSEIIAFLAFLGISEKIASDSPNCQNGPCQKFADTVIVQLGQTNLAIANEASVAVSLQQISTWGPTAGWYLVLATIPLTVLATIIVVINRFPIPVDSLGTGEAL
Ga0255308_1016056	Ga0255308_10160561	F044315	PASAMPHQQIDVAGLDGSESFRVRGVTRSRSVLMVGAVLALAVCGVLMYVNVGGSEPVDLLQQTCPDCLAKISRKISALSSDQKNMAQQFEVELDHLQHSVSMLKSEIAHVNPRSGLVRVIKSASLTPTAAPLPSPVRSSVIVNTPVDHDSEYDESGFKKGANRAKNMRDKKYGAFPNRWVDKDTVEAFTDHIYGPDPTTLDRKGVEREGYFTIAKIHSGMFYYINLEGVSNHHIKLIMQGTDGYNREYEGYGSIYTGPIYAGDHGVRDYFEVQDLVNGDVEFFRFSHPWTPPAMK
Ga0255308_1016445	Ga0255308_10164453	F090468	SMKLLEFTYTKSGGDVSKRAVIELVTPTQFVEGWDVTNLGESEFAKFTQVMGDLRRRQHEETMQLLQDFDLKHNYRRFKPEGMSDVQVEYV
Ga0255308_1016481	Ga0255308_10164811	F042749	IFAISHRPITTMIGATWMKYAAVPCLLLLAAVAVLYTDSSQQSAEPNLDVALVSSSQQTQSLALFKHAAIEDVYVYAGPEEHTRHLVPFICRLIKRHRFRLNIHQFFSVDDAPLRSVLWSHDSRVVIFPPFESYPQISAKGFTDLRAYVAAGNNVVFMGSYIALQVMNDAFGFSLRDDYKEGPYYRNDRNVRGTEFEYLPSRLNEPSPRVYGAMAKSLPPGGFSLYHSLGSVVVFYVRYDLGTIVYIGYQFDNPYQIDSWVRVIHAAMAM
Ga0255308_1016780	Ga0255308_10167801	F104576	NNLSTTEILIQQITTDEGLLAPYGVGEINLQVRYALE
Ga0255308_1017097	Ga0255308_10170971	F007472	AVGIQLGKMVYFTYKKLNICTVALLATVFAVLIAAMAVDWYSYKVEYSYTRVSASDTSLASSLYNYTQTNFDMFGQIVNVQAANTKIVRTTQQTYAQLGATSVNQQFKIQQAFVLIALLTAGLLFVAHTLYFFDGFRNKILFFVGITALRTILIIALLVVVSAEIIAFLAFLGLSDKISSDSPNCLSGPCQKFSDTVTTQLGQNFITIGNEASVAVSLQSVATWGPTAGWYLVLATIPLTVLATIIVVINRFPIPVDSLGTGEAL
Ga0255308_1017193	Ga0255308_10171932	F044315	MLALVVCGLVMFVNNGSARAQPLDLLQQTCPGCLAKISKKINALSADQQHMAQQFESELTQLQQSVSKLKAEITHVNPRSGLVRVIKSEVPKAVSPAGASEAAIAVSTQSNHDPEFDESGFKKGANRARNMRDKKYGAFPNRWVDKDTVEAFADHIYGPNPTTLDRKGVEREGYFTMAKIHSGMFYYINLEGVSNHHIKLIMQGTDGYNREYEGYGSIYTGPIYDGDHGVRDYFEVQDLVSGDVEFFRFSHPWTP
Ga0255308_1018977	Ga0255308_10189771	F101271	ADLPIEQHQGDAMRLLFDFSEAIAHQREALAKRDWAKLQQSIQALQQAMHQIASFPGGAEGVRHQLVASEGEARQTADRLIEKVAIERRSSAELIRLQLQRLQALHTMTSLDEETTTYSESGTSKGRIGRLSTWV
Ga0255308_1019332	Ga0255308_10193321	F080126	MKKVTLSLAAIALVGLYAGSASAQCAFDIAPAKGVRGSMVRNYAPCPGTENPTANTTTQGGTDACTPVTPAEVSGNGTLYSYDVKGKCDVRTSAKLVSDCAEVEDSAGNNLGLAPGACHVTYVTSKCSGILGQDGVTPAGASDDGWALATLSRATLDDPSNGDMTVIDFPVTFLYSTPDKGKIEVDSSSAQALVPLVGATGAKLPPCTQIEVVNVIIKDPAGLPFAK
Ga0255308_1023479	Ga0255308_10234791	F070881	SKIKTHTHIQILNYNTNINTIMLKKINVSKLIQNQIKSFSFVVFVTTFALIFLNSSSNIIPLNILTFMGSILLFHYYPNYYDIVISREPKLTPLLASIDFMLHYLPLIYIYIFKVFNKTEINYSLCLMMILSYVLLFHSDIRGIYFNINEYFL
Ga0255308_1025302	Ga0255308_10253021	F061852	AIGAILRLSENDIDSLTKANKSALKAIFDALANSKPL
Ga0255308_1026599	Ga0255308_10265991	F023359	VESYSTYDSNSGGMVKGYFKTSQAFVVIVLVVSFVLTVLLTLFQLDRVRNWFIFSIGMSFTRIAITLLAGLVLLSAVIAFLSFLGLPSAFKEEIPNCVDGPCREFSNSLKSPDLIEVQNGVSYSLVNTRAWGPVEGWFIVLGIIPVSVVLLALIVLNKFPLPIDSEASSGEAL
Ga0255308_1027135	Ga0255308_10271351	F051067	MSYNSLTYFDLDLKTLTPMDKFIMRAFVDESYLTNLYQATDWAQQLKMQGQTYCYRYVPQQLGLYSKMYDQNDLIDLTFISYIVPNPYTYDFSVPFNVNGHIIYVDEVDKSFIIARKGREFYESLYAALEMAKEFRANGRYYCYNMVTPNWFLPENQYAAGIPGQKLILLWYNMNYVIKN
Ga0255308_1027323	Ga0255308_10273231	F086665	EDVYVYSGKEEHTRHLVPFICRLIKRHRFRLNIHQFFSVDDAPLRSVLWSHDSRVVIFPPFESYPEISSKGFTDMRAYVAAGNNVVFMGSYIALQVMNDAFGFSLRDDYKEGPYYRNDRNVRGTEFQYLPSRLNEPSPRVYGAMAKSLPPGAFSLYDSLGSVVVFYVRYDLGTIVYIGYQFDSPYQIDNWVRVIHAAMAM
Ga0255308_1027499	Ga0255308_10274991	F051950	YISTEASYQLNFNVGFSTTRSDVLVHLAQXQYXXXFXFAFLXSFYYLVASRVVRYRSLKMRPKMSTSFRPHGKXGDFLAAVIPAIXCLNILTNSNFILRLIEXQSESSLFTIRVRARQXYXIYKFELKNFTDILTVPKNVGYNRXCVNTFGDLQVAEDYLYVLQLRSQNKXVKNYXTEVLQKTGGIKKNHIVAPQEQLRLELNNQTNINININKLSSLIIKSDNNLIN
Ga0255308_1028532	Ga0255308_10285321	F026478	RAVLLIAFVLSLLLVAECSKPKRKAVALDDEEVIQAKDFALKEIIKLSDSYRDMKIAKLISAEKARSAFSDGTNFFLKMELSCVSKCEAPLSPASTCRRGKYKGTNEIIVFWKDDGHYDGIAIDEFPQMDGEFHPCTPSYKGSEKCNPAEHRTPEQAALFMDDPEDDSVPRKEDL
Ga0255308_1030983	Ga0255308_10309831	F070194	VVFDTETSCQAFPVYDMAWRIVDRQGREYNRGSYFMPDIIERITPIFRKHEEYPEYVAKGHVEQRFYQDVKDTFNRQVKTLREKGHRILLVAYNADFDRKALKRTAELMGGSRRSRFFRFEPEWVDLWQYWAESAPRHYTSERLTENGNWKSSLEEVFRFENHNPEGYAEKHTAWSDTDDAISVLEKVIRRKKRLPVYKGDAWMPGGWRALQKRC
Ga0255308_1033591	Ga0255308_10335911	F032540	VVAVVMLAFGCAAVALAAASASTDSKLRDTSVLKVGVSDRLDVWRQYQNDEDVNPRKKKTAKADWEKQRSIPDFVDGIIGPSPTAMDEGGSKSRGSFNVKKITDKTDFYYLRITDRSGNGDRIRAELVGSDGYRLDWVGFGEIFTGPIHEGNLGETDYLRVEDLITGEEQFFRFRF
Ga0255308_1034640	Ga0255308_10346401	F027123	MATVKRYNIFCPKCNQRFPVFASLHHSIDVDSWIREKSDTHDCKAYADQLAKERSPRPINPLKASKKGA
Ga0255308_1035097	Ga0255308_10350972	F063745	MDQLIIDSINSALKPNQQPQTPTKLSPRFKALARECGFVFWGNEPHGPGPGNIDWSCDYSTEFEHYSRELVTWTCELMRQEIVRDYLFKASKNTYQHLVVQD
Ga0255308_1035108	Ga0255308_10351082	F023359	VIVLVVSFVLTVLLTLFQLDRVRNWFIFSIGMSFTRIAITLLAALVVLSSVIAFLSFLGLPQAFKDEIPNCVDGPCREFSNSIKLSDLLEVQGANTYSLVNTRTWGPVEGWFIVLGIIPVSVVLLALVVLNKFPLPIDSEASSGEAL
Ga0255308_1041837	Ga0255308_10418371	F059663	LSGILSIYFTLVFFAGFRAKIVYSWGATGARRIAMLTSLFVVISVVIAFLATTGITNALKTDNPVCVDGPCNKFVDSQTTSLGTYTHLGVVYNMNSSMTWGPAAGWFLFLACIPLSLLLTIVAGLNNYPVPIDSLGSGEAL
Ga0255308_1042429	Ga0255308_10424291	F070907	LRKMNYGSVERVSPLVRESRKPRIVALAGAAVLLGLIALGALIAQSSQPSELLGEMLARGENARNKGVPPTVFAPPPEPADWDGAKAIIGKMPPTGVAPSALQEAGFETRGSITVRRDLAAENMYFRIYGRINHHLKCTLRQYRRMSMEYQGEVIYTGFKRVYTGQSEIQTGHLPRTHDTEFD
Ga0255308_1043067	Ga0255308_10430672	F064751	MVAAAVPEAAPAVVRTVVLLEDAAGVEVAVKDVTVLAMELTDPKK
Ga0255308_1043421	Ga0255308_10434211	F080126	PVIPFQNGTGNSTTYQYTAKGKCSVSTSAKLVADCALVTDSSGAVLGLQQVPCHITYVSSKCSGIATSDETVLIDAGDIGWSLATLTRATFNDATNGDMTVIDFPVTFNYSTPSSGKMAVKSSSAEALIPLVGVNNADLPACTSLEIVNIVIKDPAGLPFAKLGGATKP
Ga0255308_1043724	Ga0255308_10437241	F025755	QITISGYAEVGMITGSAKQTRSTAHSKGLGSEFLVTVAGKGTMSNGWTFSGYQNFDTDEVGNGVDAANVNPMTTRALEISPNKDFKLFYTYDGVYGGEIARTAIPVVTERPVDLTGLSGLSEFIDVTSGGHAVGAEA
Ga0255308_1043895	Ga0255308_10438951	F025755	QMKNKVLKAIGALSVASLMSTSAFSQITISGYAEVGFITGDAEGTRGLSSSKGLGSEFLVTVAGKGTLSNGWTFTGYQNFDTDEVGNGIDTANSNPMTTRALEISPNKDFKLFYTYDGVYGGEIARTAVPVVTERPVDLTGASTLAEFIDVTSGGHAIGFDVLNVGPAGRFSVAYNPNI
Ga0255308_1044157	Ga0255308_10441572	F046147	IETTNKDPVGLGTLSDPHAIEDCVACRTIWLQVEMDVGNTALEENIYNSFSQNALEAQKSPIFYPAVHEMFNSIDDMVGDYMEGFTVNQVCENARMCR
Ga0255308_1044200	Ga0255308_10442001	F020340	FADLGLQGDAGTHFRLRFTYNTLTVDSNEFNVKPNHMHVSVHPSDGQYEAGASKADIGAGITVQARDGGNNVLTGADNTNAGSDTVVVTVLEASGSVLDANLLGSKGLFLSSGARTFADLGLQGDAGTHFRLRFTYNSLSVESNEFNVRPNHIHVSVHPSDGQYGAGASKADIGAGIT
Ga0255308_1044649	Ga0255308_10446492	F064751	VSPVSVMVIAAVPDAAPPVVRTIVVFAEVTLPEVAVNDVTPLAMEVTDPKK
Ga0255308_1046201	Ga0255308_10462011	F016245	SAGGLVQFWFGGVTNPAMNQSQVRDLAAVTTTSSGEVIGGSVTGQLPAIVNGLGNLSVGLSNTVRQGTGIRMSVSFTPVDAFPSDGRFIITLVGAGFACSDNTAVTFVSPAGGSGSVSISAGLVLTLQLSGGAFSAGGLVQFWFGGVTNPAMNQSQVRDLAAVTTTSSGEVIGG
Ga0255308_1046206	Ga0255308_10462061	F016245	NPNSNQSHISNIHAATLNAPGNIVGASVTGYLPAIVNGLGALVVGLSNTVRQGTGIVMTVSFTPVDAVPNDGRFVLTLVGAGFACSASTTVTFVSPADGSGYVSISAGLVLTLQLSGGTFSAGQAVEFVFGGVTNPNSNQSHISNIHAATLNAPGNIVGASVTGYLPAIVNGLGA
Ga0255308_1046549	Ga0255308_10465491	F016245	VTTSSTGAVVGGSVTGGLPAIVNGLGSLSVGLSNTVRQGTGIRMTVSFTPVDAVPGDGRFIMTLVGAGFACAANTAVTFVSPAGASGSVSIGPGLVLTLQLGGGVFSAGGAVQFWFGGVTNPSSDQAQVNNLAAVTTSSTGVVVGGSVTGVLPMVVNDLGFNGPTISLSTHLKA
Ga0255308_1047638	Ga0255308_10476381	F082176	SVVGVRPKYLVLGATATLELTADWPVVDDHYACVLSSGQSQSLVPLVWTGSGSVGTCAFRDTTMFETGTASLAIKHDVHEFSVDAGTLQLVPSVSVLKISPSAVEAGATSVLEIEVDADCDCRAGLECRVGQARAPAMCSAGSRTLRCAVVPVGGAGVRTFALMMDGQLLS
Ga0255308_1048942	Ga0255308_10489421	F103121	NFDIIAVVNNSVTYCFQVYATNANGNSAPSNIAEATPFNNNLPTRLWARFEPNCPSYKIEGNNVAETSYEMQRKAGVLQCPANGRLNFTKARLWSMASRNELTRKMAWASQSQLYTYDNTTNVSNQEGVGLKVVNNTLSCWTPQPPIICNSSSSSNVPGKPVVLCFSTD
Ga0255308_1048961	Ga0255308_10489611	F068878	MTEKAKKWSDATVDHLMKLVGKGRPVSADTVQHAAEELGEGFTARSVASKLRQLEVEVASMAKEKTSAFTEDEGAELAEFVVSNSGEYTYKQIAESF