NMPFamsDB

NMPFamsDB

NMPFamsDB

A database of Novel Metagenome Protein Families

A database of Novel Metagenome Protein Clusters

A database of Novel Metagenome Protein Clusters
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Sample 3300002355

3300002355: Freshwater microbial communities from Lake Mendota, WI - 20APR2010 deep hole epilimnion



Overview

Basic Information
IMG/M Taxon OID3300002355 Open in IMG/M
GOLD Reference
(Study | Sequencing Project | Analysis Project)
Gs0063444 | Gp0055839 | Ga0003597
Sample NameFreshwater microbial communities from Lake Mendota, WI - 20APR2010 deep hole epilimnion
Sequencing StatusPermanent Draft
Sequencing CenterDOE Joint Genome Institute (JGI)
Published?Y
Use PolicyOpen

Dataset Contents
Total Genome Size21641257
Sequencing Scaffolds32
Novel Protein Genes35
Associated Families31

Dataset Phylogeny
Taxonomy GroupsNumber of Scaffolds
All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Thalassiosiraceae → Thalassiosira → Thalassiosira oceanica2
All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage1
All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Oomycota → Peronosporales → Peronosporaceae → Phytophthora → Phytophthora ramorum1
Not Available22
All Organisms → cellular organisms → Bacteria → FCB group → Bacteroidetes/Chlorobi group → Bacteroidetes → unclassified Bacteroidetes → Bacteroidetes bacterium ADurb.Bin3971
All Organisms → Viruses → Predicted Viral1
All Organisms → cellular organisms → Eukaryota → Opisthokonta → Metazoa → Eumetazoa → Cnidaria → Anthozoa → Hexacorallia → Scleractinia → Astrocoeniina → Acroporidae → Acropora1
All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Skeletonemataceae → Skeletonema → Skeletonema marinoi-dohrnii complex → Skeletonema marinoi1
All Organisms → cellular organisms → Eukaryota → Opisthokonta → Metazoa → Eumetazoa → Cnidaria → Hydrozoa → Hydroidolina → Anthoathecata → Aplanulata → Hydridae → Hydra → Hydra vulgaris1
All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Thalassiosiraceae → Thalassiosira1

Ecosystem and Geography

Ecosystem Assignment (GOLD)
NameFreshwater Microbial Communities From Lake Mendota And Trout Bog Lake, Wisconsin, Usa
TypeEnvironmental
TaxonomyEnvironmental → Aquatic → Freshwater → Lentic → Epilimnion → Freshwater → Freshwater Microbial Communities From Lake Mendota And Trout Bog Lake, Wisconsin, Usa

Alternative Ecosystem Assignments
Environment Ontology (ENVO)freshwater lake biomeepilimnionlake water
Earth Microbiome Project Ontology (EMPO)Free-living → Non-saline → Water (non-saline)

Location Information
LocationLake Mendota, Madison, Wisconsin, USA
CoordinatesLat. (o)43.098333Long. (o)-89.405278Alt. (m)N/ADepth (m)N/A
Location on Map
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Associated Families

FamilyCategoryNumber of Sequences3D Structure?
F003109Metagenome / Metatranscriptome506Y
F015696Metagenome / Metatranscriptome252Y
F015817Metagenome / Metatranscriptome252Y
F018650Metagenome / Metatranscriptome234N
F020301Metagenome224N
F032577Metagenome / Metatranscriptome179N
F033356Metagenome / Metatranscriptome177Y
F034061Metagenome175N
F044498Metagenome154N
F048786Metagenome / Metatranscriptome147Y
F051784Metagenome143N
F052281Metagenome / Metatranscriptome143N
F053089Metagenome / Metatranscriptome141N
F057114Metagenome / Metatranscriptome136Y
F057117Metagenome / Metatranscriptome136Y
F057914Metagenome / Metatranscriptome135N
F060610Metagenome132Y
F061552Metagenome / Metatranscriptome131N
F069484Metagenome / Metatranscriptome124Y
F075711Metagenome / Metatranscriptome118Y
F079377Metagenome / Metatranscriptome116N
F083713Metagenome / Metatranscriptome112N
F088300Metagenome / Metatranscriptome109N
F096670Metagenome104Y
F098700Metagenome / Metatranscriptome103Y
F100269Metagenome102Y
F100374Metagenome102N
F102145Metagenome / Metatranscriptome102N
F104099Metagenome101N
F104317Metagenome100N
F104330Metagenome / Metatranscriptome100N

Associated Scaffolds

ScaffoldTaxonomyLengthIMG/M Link
B570J29638_100016All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Thalassiosiraceae → Thalassiosira → Thalassiosira oceanica9211Open in IMG/M
B570J29638_100063All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage6012Open in IMG/M
B570J29638_100297All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Thalassiosiraceae → Thalassiosira → Thalassiosira oceanica3389Open in IMG/M
B570J29638_100408All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Oomycota → Peronosporales → Peronosporaceae → Phytophthora → Phytophthora ramorum2914Open in IMG/M
B570J29638_101035Not Available1942Open in IMG/M
B570J29638_101368Not Available1711Open in IMG/M
B570J29638_101495All Organisms → cellular organisms → Bacteria → FCB group → Bacteroidetes/Chlorobi group → Bacteroidetes → unclassified Bacteroidetes → Bacteroidetes bacterium ADurb.Bin3971627Open in IMG/M
B570J29638_101771Not Available1487Open in IMG/M
B570J29638_101869Not Available1442Open in IMG/M
B570J29638_102673All Organisms → Viruses → Predicted Viral1187Open in IMG/M
B570J29638_103286Not Available1061Open in IMG/M
B570J29638_103304Not Available1057Open in IMG/M
B570J29638_103542Not Available1015Open in IMG/M
B570J29638_104048Not Available937Open in IMG/M
B570J29638_104401All Organisms → cellular organisms → Eukaryota → Opisthokonta → Metazoa → Eumetazoa → Cnidaria → Anthozoa → Hexacorallia → Scleractinia → Astrocoeniina → Acroporidae → Acropora892Open in IMG/M
B570J29638_104432Not Available887Open in IMG/M
B570J29638_105096Not Available817Open in IMG/M
B570J29638_105525Not Available776Open in IMG/M
B570J29638_106017All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Skeletonemataceae → Skeletonema → Skeletonema marinoi-dohrnii complex → Skeletonema marinoi737Open in IMG/M
B570J29638_107183Not Available656Open in IMG/M
B570J29638_107285All Organisms → cellular organisms → Eukaryota → Opisthokonta → Metazoa → Eumetazoa → Cnidaria → Hydrozoa → Hydroidolina → Anthoathecata → Aplanulata → Hydridae → Hydra → Hydra vulgaris651Open in IMG/M
B570J29638_107342Not Available647Open in IMG/M
B570J29638_107535Not Available637Open in IMG/M
B570J29638_107666Not Available630Open in IMG/M
B570J29638_108532Not Available589Open in IMG/M
B570J29638_108652Not Available584Open in IMG/M
B570J29638_109055Not Available567Open in IMG/M
B570J29638_109139Not Available564Open in IMG/M
B570J29638_110146Not Available529Open in IMG/M
B570J29638_110258All Organisms → cellular organisms → Eukaryota → Sar → Stramenopiles → Ochrophyta → Bacillariophyta → Coscinodiscophyceae → Thalassiosirophycidae → Thalassiosirales → Thalassiosiraceae → Thalassiosira525Open in IMG/M
B570J29638_110510Not Available518Open in IMG/M
B570J29638_110946Not Available504Open in IMG/M

Sequences

Scaffold IDProtein IDFamilySequence
B570J29638_100016B570J29638_1000163F061552MKDEEEMKRGVHITGTITMEATHHHPTCLHXLTTLVNEKITGFDCPILDISDSKMGKVGLAILVDMSPLMAPARECDGYLKLTRKNLKRWKQPSIYAWPIVNSGLKRIDGVGLLILNDLAKSVYNFITTVQSEGNGXGSANRS*
B570J29638_100063B570J29638_1000633F034061VKNKTAMCYESLLGLQGCDRPEPTTGLYIDDLGINQTLLGQLITDQYNSGVELFEAKRAFAWRKMSTDILSRLTPMMKADTVVESKRIGQVVSNAANIDTMVGAGKYTGIRVTIDPNTESFLNFYLSNFKIDIYTMAVPVEIFVYDMTTLKLIDSFFYQSEAVEQFIGKTFKANRRKLDLAFVYESLYDTTKMIPKKGHCFDCSGNVRGAHICPFVDAVGIELTVSGDDVISSKSKKYTQGMSLVYNVNCDREAWLCSIGGLMAMPLAYATAVEIYNYGLTISPNQRVNTTVSVNTGFATSDPNDGMIAGRDIAATRYSEELTAMLQNMRLPSDNTCFDCRRNMKYVTALP*
B570J29638_100297B570J29638_1002973F044498MQTDLNKFSYVANNERVLLLSRYSTNCSLTELLELQSPMAEIESETVPILSFLNGAIQRPLSYEQHIMNQKLMCAKKKFEKSCLLAYRVYCDMELKHNLLVKQYCNDEMILCSKAHDHHGYHCKPSNENHSTFEYDVKKTCETNIVFDGLHDLRCIMAPDYYPDGSHYIFPTPILSCLDSVNEEDVRLLLSLRH*
B570J29638_100408B570J29638_1004083F096670MLWGQRITVYTDHKNLTRDGLGLTSDRVARWRILLEEYAPEIIYIKGIHNTVADAISRLDYDPKLNTTNEYNHATHVKSTKVVSNHQWMMFSKLWSCFNETQDPDDVNPIEMNQVFANRSEEDEIFPLTVKEIVEAQKADPIL
B570J29638_101035B570J29638_1010352F104099MLRRREQLLTVDCTAYRAVQLNYNDRLTVEYNINIPAFVIHNPDLRRNALERVRVLLETDFNPTDTIDYXITGTYVLRNTENDDTREWVGSFYAGLHNPAVLQDFQLXNSRSFVNTVXNLLETCDEQFLYNGRDTKWEFDSLQSIIINVQSTVVEDHRVIQERRLEQGRRRRKFRQTFDLA*
B570J29638_101368B570J29638_1013684F033356MTTQLTNNSSINPLQDDILPQEDQRPKTHLQLQYITQTNRLPFTEAHQEQYSANVTINPFKLLELHNKLIVLEGQLSHLTDKVAAIVVEIHKMTHQN*
B570J29638_101495B570J29638_1014951F100374EELGIEYFQYLGGEIPTTRPFCEHREGEIYHRKEIEAWGNGENSAGINDIRNGTWAGRIEGTDSRSIFTFVGGWNCRHFLVPVIKQRVPASVIARAEAEGYATPTRPRGGAEALTA*
B570J29638_101771B570J29638_1017711F051784MSNVDEDEILELEISDDETFGLCGVGSPGKKRKVAEEVLDTGVLVERTVTLLSNSTVPITLPTSKQTLSKNSVESIPWDDTEIPWDDTEIPCDVSSSASVPAKLSFTQY
B570J29638_101869B570J29638_1018694F033356MTNQFTSNSSLNPLQEDILPQEDQRPKTHLQLQFIKQTNRLPFTEAHQEQYSANVTINPFKLLEIHNKLIILEGQLSHLTDKVAAIVVEIHKMTHQN*
B570J29638_102673B570J29638_1026733F060610MTMDEGRDSTYTTIDEGCVVGIGCKVHTHHHTIHIEPQIVYQSMEKFTIFGRNYCTNQWGQTYELPAEEPMLEPQPMQQTYASDTITPSSSAFLLAPKPXAKIIIKPRTEFTEYKPTMDGPIMGMLLTFTIYLTVQWAWSSMGAWNNLYSELSACLRSSS*
B570J29638_102807B570J29638_1028073F100269MASVRTETIYLIAIIVLVFLLLKSCGENVSNDYRLKHTIYEDSILIASQKKIIAQSSSDAAKQAQQIAELEVKVKNASEVVRIETRTIIKTQIKLGDTVMVQGKPYIQLPKPFLKQTEWYTIGGMINRLGWLQIDSLVIPAKFTYAVGDTMRTGFINRIFRKSDQVVRMRVDNPNVTITGMDNIYIKQDKKWHQTTAFKVGVGVLIGVAITSVGNK*
B570J29638_103286B570J29638_1032861F052281CNKYRLAGDTALWGTRRQSMSTGGTTPGEKCPGSLIVKHEFGMSGGDHPYIKVEHKCVDNREVTSAVARGVKDYRPQLERISPNIEHGNFLSRKVIEEVLRELKNAEKNDKNLWQNITGGNTVRKWIPDMYDKNVKRVALKVIVETALKGYITIVQGRYPTLKYFKVGALKSLPGAKSQYVGHGGRLHSDYPQSVEELEPRFRPVSIIVGLNSFNFMWLNDRTSRESEIRKMTVYPGEMIMFTNHCLHAGGENSTNEEQTRLFAYLASDESHFPSGEVTTWDWQRRDNNPLISKPSRNANTAIVRRPGTNQIRLISGRVASVGGVPDRG*
B570J29638_103304B570J29638_1033042F032577MHIANNYNDVVDVITSLIAIYARTISVEPTIQQAINKRQEHTQIASKLYHSLLPMLYGCNAIELSDLQAHYNDLKRTISRSFAVDLEHPRAHRAFDPNP*
B570J29638_103542B570J29638_1035422F057117RTEAPKRDSTVPPSDGGNERAPANQRLKKPRRSAVADSSKRNVSDMGMFFLHKPDMKASDIFPKDMSELVCVDFTCKGRECTKENCPHLHPRKVGDMKKETVTTIGEHFLAKKIGWFNEWHFLKVQHLLSDKFKALMGGKDGCGSSKKD*
B570J29638_104048B570J29638_1040481F079377VYVQTAGPTDFQNVSRISFLPANFTIDEDNFSSITENSLVVLDDFSFKLANNKQAKINFLKVVNYILVIHNLV*
B570J29638_104401B570J29638_1044011F020301YHCLEAPHFTRDCKNHEGRKCGIDGCTLYHHRVLHRDPKSSRFVGYQIDDEMEPDPPTAEELADVQNAFKISQNGAISIQTLICNVLSGKNKRAANIRTVVLIDSGSSVTFIDEDFAKENNFRVLGKRKGMTLHMLERVETLPGEQLHVEFQLSSICDHVQSCTKNVSGWTVKNLAEHTSVVDWSEQKKQFAHLKDLHFPKMPEDSTIKIIMGVDNTAFFTPVETIRNPDNETDPTAIRLSLGWTCVGRSSPSPKNDKKLSFIFKNIIFKPKN*
B570J29638_104432B570J29638_1044322F057114AHIIPRTPTFTSFGDSCLEGAGGYCISLGFWWHIPFPEAVIQRTLKHKKDNKDGLLISINVLEFVTVIVNYCAALHFVTTTSATDDPYPVLLNVTDNASALSWTTGACKKSKVGRLLARFFCSLMINSPLGINSKWISTDDNKIADDISRIKRGSATEDSPPFFDYSTLTQTYPELIHCSSFKIQPELISLIWEIVLTERWPCHDEIRKLRLKPLGRLITSSGD*
B570J29638_105096B570J29638_1050961F104099MKIGSSHILKFLFYFSENYIKPTSREEKKTSVKMIRRREQLLTVDCTAYNSVQSNQHRDFYLVEYNINIPAFVVYNREDRWNALERIRVLLETDFNPTDEIDYQITGTYVLRNTENNDTREWVGSFYAGLHNPAIIQDFQLFDRHSFVNTVHLSLENVDEKLLYNGRD
B570J29638_105525B570J29638_1055251F088300CGLSNDGTCIRFPIFRLGCCGEDDWVADVAEIPVPTVLEKLFFEKVARLIQYTCDCNPEYSVEAFDWDWASFHKNILLPNRMHIRSVMQLSDTITDRVVYSLHILLNEWNGDDEEENDEDVNVRFEKFFSVEDLDF*
B570J29638_106017B570J29638_1060171F098700PNNMAGILINNLIKEEDIARQRSPLDSKMYTEMLRRSNASLSLDSEHRTLFDATTIGRYLGPRVSEFAQTTDKTVDYHVYPSGRKVIKAFIANDFQFIDVTGQVITGLTDTSIDVVDRVRVTWRIQKNRQNNQKVTLSCDKANPTICPVLAALRLVLRARRLSQPDSMPVACYLKKDTLAYLTGSRIALHFRAAAKAVRPNISKDEEQRYSAHSLRVWACVLLDEAGKSPDYIKKRLRWMGDSFR
B570J29638_107183B570J29638_1071831F102145MDTEQQKSSETLLYNFDYPMVTECGAVKITADRVILQFPEKPTVYVENSVTLRRNVTLRIENFPGFSIDRIPIHTGTDFFRVKEMIEKCLQSYSYLISKTYFSPHSDWFNSIKYYKTLSTEFFTSNTFHLSCFEHTFITCIELSVINKECDEKEIPLHFQLCIGKHVVFCTERDLKCVTQAETVHF
B570J29638_107285B570J29638_1072851F020301SQNGAISIQTLICNVLSGKNKRAANVKTVVLIDSGSSVTCIDEDFALEHNLRILDKREGMTLHMLERIVKLPEDQYHVELQLSSVEQGCTKNISAWTVKNLARHTSVVDWSERKKQFPHLKDINFPTMPEDSTIKIIMGVDNTALYAPVKIVPNDDNDKDPIAIKLSLGWTCVGRSSPSPYKNEVFTNVVFRPKN*
B570J29638_107342B570J29638_1073421F053089DSWYIFVPDIIENHLLSHVIPAPVDISNDLQNKHMIEDFFVYSPVDIIYKDPSSVFWIHPFVDFAMNRSTGNVGSWNKLLFMFTEFCLNNTTYFTRFSDSIIGINVNTSLTSLFDFKFFHLSQVETLLQQITKFLGRKNSMIQSCHFITHNPTFDYITSIHPNVFTFIDYIINNHNDMMPDFKTGIFNI*
B570J29638_107535B570J29638_1075351F104330SPKGQTLNIKCPSRQNHKPTEFIPKGISKFRLPAGCRTELEDHFVYSDSSISSDSGLEHIKLPDVVSLNIPDVSPEHLETIMSDMIKDGLYRPTMNDIIEAHEHMEDLQHHSFRSMIVWIIFAIVILFLIAFTIYIFQYLYYIRATIYSVLKLKSRKALYTFISTFLSTQTLHMPPVQNQPA*
B570J29638_107666B570J29638_1076661F069484MAEACYTARATTKQQVLDGIDKLTLQTNEHGQYTHDAXLLLQKGIQRVLVSQFNPSDPGKGMVKLLDDGDNILTRIQSTDAITAGSVLKSAKKRADDLSTTTGKTILPTFTTRTEAQEEADRLNVINQSVIGAKEGVVEAVSKLVGSDITDAILRTADGSDFKSIDDYTLYEVMKVAIDGADRPTTNDVLEQLLEVI
B570J29638_108532B570J29638_1085321F018650IVMDEYFSLEELRYQVMSILRHAAQFPNNSDVIVLEDQELQMVFDSWYIFVPDVVENHLLSHVIPAPADISNDLQNKHMTEDFYVDSPVDLIYKDPSSVFWIPSFVDFAMNQSTGNVGSWNKLLFMFTEFCLNNTTYFTRFSDCIIGINENTCLTSLFDFKYFHISQIETLLQKITKFLGRKNSIFQSCHFITHNP
B570J29638_108652B570J29638_1086521F015817GLRRKSMASLWECIDVHKLTVYSVDAAERQRFYMLCNLKKPAKSSIRSHVTRMETLNKYLGLLPTIKNSPQAVASTELGNVPFNETTLASIVLSHLPVAWRTQYALIRVLVPESPRASLVDFENIEKLLAEKANEAARANKAKVATALNRANEHVPRKGKREHGGGPSKGAPKKGRTAKYCKWCKAVDGPFTTH
B570J29638_109055B570J29638_1090551F015696AKGAPQTKSDMVTPVKAGGGTLQKXSPSPNQKKKDERNKPKIDNLMHPNGTCYGWAFHNFYAAKEELKRISNKLGAITVIGSEELKPFSNLTTKWLKEAEYSNYIWVIRIDLEQDGAGHCFPVEAHEAYGKKIARGVISQGIWGKGEVEVKMATLSHAEALDLDVRFSVVHNQAADDAFDEAIREADS
B570J29638_109139B570J29638_1091391F104330KGQTLNIKCPSRQNHKPTEFIPKGISKFRLPAGCRTELEDHFVYSDSSISSDSGLEHIKLPDVVSLNIPDVSPEYLETIMSDMIKDGLYRPTMNDIIEAHEHMEDLEHHSFRSMIVWIIFAIIILFLIAFTIYIFQYLYYIRATIYSVLKLKSRKALYTFISTFLSAQILNTPPIQNHPA
B570J29638_110022B570J29638_1100221F104317KEYPFTNIDELQHVEAHVKVNQGDIFILQDQSSRTRDMYDLLIRPLENLFACDMPMSEDLLLAQNDVIKQKWIFSVITKISGHCHWPKKSTMFILRELSKIRILNQKALTETFDQNWNPVSRKCPMQMGAKTTPEDIFDIAENKWNVNINDFNPGGRLYGIYVKMDEPLPFHIQHRF
B570J29638_110048B570J29638_1100481F057914YKNKYYILNQLPTIFPLLLRNVNPQDQLTYKRIPVRADQILDIVQGRTDHLNFPFTINIYTAYSYTGKASNEIRTNMIGQLLAKKNTEEIFHVFLTPDIEQRNVIKINALENVKHSAKFDKHDLFTNTLITEGDKTVFKRSPKEVLLNQKNCICEHKETQDYSPLSRDRNLGIEQLL
B570J29638_110146B570J29638_1101461F075711GSQKYIPTLKRAVPQSWGTSNGTFKTNKVGEITLSFAEYSLSKSVHLTPDIVEYDAGATAPLYDLIXGKXTLHDIGAVLDFKEKTITIDGILLPMRNIVNLQLKPSVTRALRHNTCQAQEPISTQTATKRVIKILDAKYDKADLPAIVNDNCSHLTPLQREKLLSLLLKYESLFDG
B570J29638_110258B570J29638_1102581F003109MHQTVGNVLRTLLHSEPPQNITTAKEFVDEALSIATHAMRAGIHTTLGSSPGSLVFNRDMFLNIPLIADWHAITLKREHLIHENLLRENQKRRRYDYMPQQRVLKKRWKPRKLDERTSGLYRVVQTHVNGTVTIE
B570J29638_110510B570J29638_1105101F083713SLYHSCQDVLTPYAILVKIALEITGLRGQAENEHENIFITMNEALELCLSKAPADIRNPNNGSLARVVEGAKLINWINDEAVEHCTPFQFNSHALVQRIRRARADLVESHVNELRWDEIQTLALHHLDLLNGRMPFSNELKFVDLRFHIMDHCAHCGSLGHRSEACVERTFP
B570J29638_110946B570J29638_1109461F048786GGEPIVCSSYQQSAIAHGYVDSIQDVTLTYDDMCGNGTASQCRSYFVVLTVHGYATHAIFDDYERRRFMFMDYITYRGVPELIAEQMMLQDLERHFRKSHSSLRKYGFPVPDGVPTELEEARSVWLSPEVQTRQGQLLDSLNLTQPNNSEQQLAFDSIMNSILGFRDA

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