Metagenome / Metatranscriptome Family F071157

Overview

Basic Information

• Family ID: F071157
• Family Type: Metagenome / Metatranscriptome
• Number of Sequences: 122
• Average Sequence Length: 84 residues
• Representative Sequence: MSYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN
• Number of Associated Samples: 74
• Number of Associated Scaffolds: 122

Quality Assessment

• Transcriptomic Evidence: Yes
• Most common taxonomic group: Unclassified
• % of genes with valid RBS motifs: 55.74 %
• % of genes near scaffold ends (potentially truncated): 22.13 %
• % of genes from short scaffolds (< 2000 bps): 54.10 %
• Associated GOLD sequencing projects: 71
• AlphaFold2 3D model prediction: Yes
• 3D model pTM-score: 0.57

Note: High quality evidence is represented by blue. Low quality evidence is represented by red.

Most Common Taxonomy

• Group: Unclassified (50.820 % of family members)
• NCBI Taxonomy ID: N/A
• Taxonomy: N/A

Most Common Ecosystem

• GOLD Ecosystem: Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater (26.230 % of family members)
• Environment Ontology (ENVO): Unclassified (72.951 % of family members)
• Earth Microbiome Project Ontology (EMPO): Free-living → Non-saline → Water (non-saline) (81.148 % of family members)

Structure & Topology

Predicted Secondary Structure and Topology

Predicted Topology & Secondary Structure

• Classification: Globular
• Signal Peptide: No
• Secondary Structure distribution:
  α-helix: 42.98%
  β-sheet: 7.89%
  Coil/Unstructured: 49.12%

Predicted 3D Structure

Low Quality Model:

This family has a low confidence model (pTM < 0.7) and has not been screened against SCOPe or PDB.

Gene Neighborhood

Neighboring Pfam domains

Pfam ID	Name	% Frequency in 122 Family Scaffolds
PF02223	Thymidylate_kin	19.67
PF00011	HSP20	16.39
PF08761	dUTPase_2	11.48
PF02016	Peptidase_S66	9.02
PF02511	Thy1	4.10
PF00149	Metallophos	3.28
PF14279	HNH_5	3.28
PF13539	Peptidase_M15_4	3.28
PF00856	SET	1.64
PF00154	RecA	1.64

Neighboring Clusters of Orthologous Genes (COGs)

COG ID	Name	Functional Category	% Frequency in 122 Family Scaffolds
COG0125	Thymidylate kinase	Nucleotide transport and metabolism [F]	19.67
COG0071	Small heat shock protein IbpA, HSP20 family	Posttranslational modification, protein turnover, chaperones [O]	16.39
COG4508	Dimeric dUTPase, all-alpha-NTP-PPase (MazG) superfamily	Nucleotide transport and metabolism [F]	11.48
COG1619	Muramoyltetrapeptide carboxypeptidase LdcA (peptidoglycan recycling)	Cell wall/membrane/envelope biogenesis [M]	9.02
COG1351	Thymidylate synthase ThyX, FAD-dependent family	Nucleotide transport and metabolism [F]	4.10
COG0468	RecA/RadA recombinase	Replication, recombination and repair [L]	1.64
COG0209	Ribonucleotide reductase alpha subunit	Nucleotide transport and metabolism [F]	0.82
COG0258	5'-3' exonuclease Xni/ExoIX (flap endonuclease)	Replication, recombination and repair [L]	0.82
COG0587	DNA polymerase III, alpha subunit	Replication, recombination and repair [L]	0.82
COG0863	DNA modification methylase	Replication, recombination and repair [L]	0.82

Phylogeny

NCBI Taxonomy

Level 1

Name	Rank	Taxonomy	Distribution
All Organisms	root	All Organisms	49.18 %
Unclassified	root	N/A	50.82 %

Level 2

Name	Rank	Taxonomy	Distribution
cellular organisms	root	All Organisms → cellular organisms	45.08 %
Unclassified	root	N/A	50.82 %
Viruses	superkingdom	All Organisms → Viruses	4.10 %

Level 3

Name	Rank	Taxonomy	Distribution
Archaea	superkingdom	All Organisms → cellular organisms → Archaea	23.77 %
Bacteria	superkingdom	All Organisms → cellular organisms → Bacteria	21.31 %
Duplodnaviria	clade	All Organisms → Viruses → Duplodnaviria	3.28 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Unclassified	root	N/A	50.82 %

Level 4

Name	Rank	Taxonomy	Distribution
Bacteria incertae sedis	no rank	All Organisms → cellular organisms → Bacteria → Bacteria incertae sedis	4.10 %
DPANN group	clade	All Organisms → cellular organisms → Archaea → DPANN group	22.95 %
Heunggongvirae	kingdom	All Organisms → Viruses → Duplodnaviria → Heunggongvirae	3.28 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Proteobacteria	phylum	All Organisms → cellular organisms → Bacteria → Proteobacteria	4.92 %
Unclassified	root	N/A	50.82 %

Level 5

Name	Rank	Taxonomy	Distribution
Bacteria candidate phyla	clade	All Organisms → cellular organisms → Bacteria → Bacteria incertae sedis → Bacteria candidate phyla	4.10 %
Candidatus Pacearchaeota	clade	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota	22.95 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Proteobacteria	phylum	All Organisms → cellular organisms → Bacteria → Proteobacteria	4.92 %
Unclassified	root	N/A	50.82 %
Uroviricota	phylum	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota	3.28 %

Level 6

Name	Rank	Taxonomy	Distribution
Candidatus Dependentiae	clade	All Organisms → cellular organisms → Bacteria → Bacteria incertae sedis → Bacteria candidate phyla → Candidatus Dependentiae	4.10 %
Candidatus Pacearchaeota archaeon	species	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	22.95 %
Caudoviricetes	class	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes	3.28 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Proteobacteria	phylum	All Organisms → cellular organisms → Bacteria → Proteobacteria	4.92 %
Unclassified	root	N/A	50.82 %

Level 7

Name	Rank	Taxonomy	Distribution
Candidatus Pacearchaeota archaeon	species	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	22.95 %
environmental samples	no rank	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples	2.46 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Proteobacteria	phylum	All Organisms → cellular organisms → Bacteria → Proteobacteria	4.92 %
Unclassified	root	N/A	50.82 %
unclassified Candidatus Dependentiae	no rank	All Organisms → cellular organisms → Bacteria → Bacteria incertae sedis → Bacteria candidate phyla → Candidatus Dependentiae → unclassified Candidatus Dependentiae	4.10 %

Level 8

Name	Rank	Taxonomy	Distribution
Candidatus Dependentiae bacterium ADurb.Bin331	species	All Organisms → cellular organisms → Bacteria → Bacteria incertae sedis → Bacteria candidate phyla → Candidatus Dependentiae → unclassified Candidatus Dependentiae → Candidatus Dependentiae bacterium ADurb.Bin331	4.10 %
Candidatus Pacearchaeota archaeon	species	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	22.95 %
Predicted Viral	clade	All Organisms → Viruses → Predicted Viral	0.82 %
Proteobacteria	phylum	All Organisms → cellular organisms → Bacteria → Proteobacteria	4.92 %
Unclassified	root	N/A	50.82 %
uncultured Caudovirales phage	species	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage	2.46 %

Associated Scaffolds

Scaffold	Taxonomy	Length
(restricted) 3300013126|Ga0172367_10078473	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	2423
(restricted) 3300013126|Ga0172367_10110652	All Organisms → Viruses → Duplodnaviria → Heunggongvirae → Uroviricota → Caudoviricetes → environmental samples → uncultured Caudovirales phage	1907
(restricted) 3300013126|Ga0172367_10238130	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	1115
(restricted) 3300013131|Ga0172373_10002539	Not Available	27739
(restricted) 3300013131|Ga0172373_10007776	Not Available	13798
(restricted) 3300013131|Ga0172373_10118800	All Organisms → cellular organisms → Bacteria	1948
(restricted) 3300013131|Ga0172373_10234301	Not Available	1223
(restricted) 3300013132|Ga0172372_10124973	All Organisms → cellular organisms → Archaea → DPANN group → Candidatus Pacearchaeota → Candidatus Pacearchaeota archaeon	2098
(restricted) 3300014720|Ga0172376_10270514	Not Available	1031
3300000929|NpDRAFT_10225923	Not Available	1212

Note: Some of these datasets are restricted, as per the data usage policy of the Joint Genome Institute (JGI). Utilizing any of their features below requires obtaining a license from the datasets' corresponding author(s).

Environmental Properties

Associated Habitat Types

Ecosystem

Habitat	Taxonomy	Distribution
Environmental	Environmental	100.00%

Class

Habitat	Taxonomy	Distribution
Aquatic	Environmental → Aquatic	96.72%
Terrestrial	Environmental → Terrestrial	3.28%

Category

Habitat	Taxonomy	Distribution
Deep Subsurface	Environmental → Terrestrial → Deep Subsurface	3.28%
Freshwater	Environmental → Aquatic → Freshwater	83.61%
Marine	Environmental → Aquatic → Marine	13.11%

Type

Habitat	Taxonomy	Distribution
Coastal	Environmental → Aquatic → Marine → Coastal	9.02%
Ice	Environmental → Aquatic → Freshwater → Ice	0.82%
Intertidal Zone	Environmental → Aquatic → Marine → Intertidal Zone	1.64%
Lake	Environmental → Aquatic → Freshwater → Lake	67.21%
Lentic	Environmental → Aquatic → Freshwater → Lentic	8.20%
Neritic Zone	Environmental → Aquatic → Marine → Neritic Zone	0.82%
River	Environmental → Aquatic → Freshwater → River	6.56%
Unclassified	Environmental → Terrestrial → Deep Subsurface → Unclassified	3.28%
Unclassified	Environmental → Aquatic → Marine → Unclassified	1.64%
Unclassified	Environmental → Aquatic → Freshwater → Unclassified	0.82%

Subtype

Habitat	Taxonomy	Distribution
Epilimnion	Environmental → Aquatic → Freshwater → Lentic → Epilimnion	1.64%
Estuary	Environmental → Aquatic → Marine → Intertidal Zone → Estuary	1.64%
Unclassified	Environmental → Aquatic → Freshwater → Lake → Unclassified	67.21%
Unclassified	Environmental → Aquatic → Marine → Coastal → Unclassified	9.02%
Unclassified	Environmental → Aquatic → Freshwater → Lentic → Unclassified	6.56%
Unclassified	Environmental → Aquatic → Freshwater → River → Unclassified	6.56%
Unclassified	Environmental → Terrestrial → Deep Subsurface → Unclassified → Unclassified	3.28%
Unclassified	Environmental → Aquatic → Marine → Unclassified → Unclassified	1.64%
Unclassified	Environmental → Aquatic → Freshwater → Unclassified → Unclassified	0.82%
Unclassified	Environmental → Aquatic → Freshwater → Ice → Unclassified	0.82%
Unclassified	Environmental → Aquatic → Marine → Neritic Zone → Unclassified	0.82%

Habitat

Habitat	Taxonomy	Distribution
Aqueous	Environmental → Aquatic → Marine → Coastal → Unclassified → Aqueous	4.92%
Deep Subsurface Sediment	Environmental → Terrestrial → Deep Subsurface → Unclassified → Unclassified → Deep Subsurface Sediment	0.82%
Estuarine	Environmental → Aquatic → Marine → Intertidal Zone → Estuary → Estuarine	1.64%
Estuarine	Environmental → Aquatic → Marine → Unclassified → Unclassified → Estuarine	1.64%
Fracking Water	Environmental → Terrestrial → Deep Subsurface → Unclassified → Unclassified → Fracking Water	2.46%
Freshwater	Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater	26.23%
Freshwater	Environmental → Aquatic → Freshwater → River → Unclassified → Freshwater	6.56%
Freshwater	Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater	5.74%
Freshwater	Environmental → Aquatic → Freshwater → Lentic → Epilimnion → Freshwater	1.64%
Freshwater And Marine	Environmental → Aquatic → Marine → Neritic Zone → Unclassified → Freshwater And Marine	0.82%
Freshwater Lake	Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater Lake	18.85%
Freshwater Lake	Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater Lake	12.30%
Freshwater Lake	Environmental → Aquatic → Freshwater → Lentic → Unclassified → Freshwater Lake	5.74%
Freshwater Lentic	Environmental → Aquatic → Freshwater → Lentic → Unclassified → Freshwater Lentic	0.82%
Freshwater To Marine Saline Gradient	Environmental → Aquatic → Marine → Coastal → Unclassified → Freshwater To Marine Saline Gradient	4.10%
Freshwater, Plankton	Environmental → Aquatic → Freshwater → Lake → Unclassified → Freshwater, Plankton	0.82%
Lake	Environmental → Aquatic → Freshwater → Lake → Unclassified → Lake	0.82%
Lake Water	Environmental → Aquatic → Freshwater → Lake → Unclassified → Lake Water	2.46%
Surface Ice	Environmental → Aquatic → Freshwater → Ice → Unclassified → Surface Ice	0.82%
Water Bodies	Environmental → Aquatic → Freshwater → Unclassified → Unclassified → Water Bodies	0.82%

Associated Samples

Note: Some of these datasets are restricted, as per the data usage policy of the Joint Genome Institute (JGI). Utilizing any of their features below requires obtaining a license from the datasets' corresponding author(s).

Taxon OID	Sample Name	Habitat Type
3300000929	Marine plume microbial communities from the Columbia River - 15 PSU	Environmental
3300002835	Freshwater microbial communities from Lake Mendota, WI - (Lake Mendota Combined Ray assembly, ASSEMBLY_DATE=20140605)	Environmental
3300005662	Freshwater lake microbial communities from Lake Michigan, USA - Su13.BD.MLB.SD (version 4)	Environmental
3300005758	Cyanobacteria communities in tropical freswater systems - freshwater lake in Singapore	Environmental
3300005805	Microbial and algae communities from Cheney Reservoir in Wichita, Kansas, USA	Environmental
3300006641	Aqueous microbial communities from the Delaware River and Bay under freshwater to marine salinity gradient to study organic matter cycling in a time-series - DEBay_Sum_0.19_D_>0.8_DNA	Environmental
3300006917	Aqueous microbial communities from the Delaware River and Bay under freshwater to marine salinity gradient to study organic matter cycling in a time-series - DEBay_Sum_0.19_N_<0.8_DNA	Environmental
3300007177	Combined Assembly of cyanobacterial bloom in Marina Bay water reservoir, Singapore (Diel cycle-Surface and Bottom layers) 16 sequencing projects	Environmental
3300007541	Freshwater to marine saline gradient viral communities from Chesapeake Bay - CB_1508_1S Viral MetaG	Environmental
3300007542	Freshwater to marine saline gradient viral communities from Chesapeake Bay - CB_1504_1 Viral MetaG	Environmental

Family Sequences

Note: Some of these sequences are restricted, as per the data usage policy of the Joint Genome Institute (JGI). Utilizing any of their features below requires obtaining a license from the datasets' corresponding author(s).

Protein ID	Sample Taxon ID	Habitat	Sequence
NpDRAFT_102259231	3300000929	Freshwater And Marine	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN*
B570J40625_1003247231	3300002835	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLQDAEKLKTQTESTSELPKLIFEN*
Ga0078894_100227957	3300005662	Freshwater Lake	MSYKISDAVAMRMIQIFQEALLLGLDGADLMRQVRLVVDDTDPGTVTLDPQYEKQVIEMHEKYLQDAEKLKSQSTGTEGPKLIFEN*
Ga0078894_102913703	3300005662	Freshwater Lake	MSYKISDAVAMRMIQIFQEALLLGLDGADLMRQIRLVVDSENTDVLTLDPQYESQVAEMHKKYLQEAENLKTRSSGDDVFKLTFES*
Ga0078894_115332781	3300005662	Freshwater Lake	MSYKISDAVSMRMIQIFQEALLLGVDGADLMRQVRLVVDENNVDTLTLDPAYEKQVADMHTKYLAEAESLKENSLKEQEQLLVFEN*
Ga0078117_10117845	3300005758	Lake Water	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLVVDEKNTDTLTLDPNYEKQVADMHAKYLAEAETLKQKSLADQDQFKLVFEN*
Ga0078117_10117855	3300005758	Lake Water	MSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDERNTDTLTLDPSYEKQVADMHAKYLAEAETLKQKSLADQDQFKLVFEN*
Ga0078117_10489812	3300005758	Lake Water	MSYKISDSVAMRMIQIFQEAVLLGLDGADLMRQVRLVVDTSNPDTVTLDPTYESQVAEMHKKYLSDAEKIANEKLKTPNVIS*
Ga0079957_100302810	3300005805	Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLTVDEANPDTITLCPEYEKQVAEMHKKYLEDAEKLKNQTDAAELINTRIVLDS*
Ga0075471_101100573	3300006641	Aqueous	MNYKISDSVAMRFIQIFQEAVLMGVDGADLMRQVRLTVDESSPDTLTLHPEYEKMVEAQHKKYLEDAEKLKSQSETLTDVPKLIFEN*
Ga0075472_101056381	3300006917	Aqueous	ISDSVAMRFIQIFQAAVLMGVDGADLMRQVRLTVDESSPDTLTLHPEYEKMVEAQHKKYLEDAEKLKSQSETLTDVPKLIFEN*
Ga0102978_10577976	3300007177	Freshwater Lake	MSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDPDQPDTITLDPVYEQQVHSMHKKYLEEAEMLKSKLNDNSESSKLIFES*
Ga0099848_10260672	3300007541	Aqueous	MSYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVVDESTPDTVSLCPEYEKMVESQHRKYLDDAEKLKTVNESNGIPKLIFES*
Ga0099848_11248691	3300007541	Aqueous	FIQIFQEAVLLGVDGADLMRQVRLVVDETTPDTVTLCPEYEKQVTEMHKKYLEEAEKLSTVSESSRPGLIFEN*
Ga0099846_10444412	3300007542	Aqueous	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDETTPDTVTLCPEYEKQVTEMHKKYLEEAEKLSTVSESSRPGLVFEN*
Ga0102919_12251881	3300007597	Estuarine	KISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLIFEN*
Ga0110929_11456621	3300008072	Water Bodies	MRMIQIFQEALLLGVDGADLMRQVRLVVDSENSDTLTLDPQYVDQVNQMHQKYLAQAESLKASETNQLKIIFDNN*
Ga0114341_1000163140	3300008108	Freshwater, Plankton	MSYKISDSIAMRMIQIFQEAVLLGVDGADLMRQVRLVVDESQPDTMTLDPQYEKQVAEMHEKYLKESEHLKEKTESRSQKLLFES*
Ga0114973_100268481	3300009068	Freshwater Lake	SYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN*
Ga0114973_101061144	3300009068	Freshwater Lake	SYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN*
Ga0114973_106120521	3300009068	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDESNSDTVTLCPQYESQVLEMHKKYLEDLEKLKSQTEMSNSVNTKIIFDS*
Ga0114980_1000069220	3300009152	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN*
Ga0114980_1000346111	3300009152	Freshwater Lake	MSYKISDSVAMRMIQIFQEALLLGLDGADLMRQVRLVVDSSAPDVLTLDPQYESQVSEMHQKYLDEAEKLAQASSREVLVFER*
Ga0114968_101137792	3300009155	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDETNPDTVVLCPQYENQVLEMHKKYLEDAERLRARSESITTETPRLILEN*
Ga0114969_1001037813	3300009181	Freshwater Lake	MYGFIMSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDETNPDTVVLCPQYENQVLEMHKKYLEDAERLRARSESITTETPRLILEN*
Ga0114974_105516722	3300009183	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEKDTDTVTLCPTYEKQVVEMHKKYLDDVEKLTAVSESSRPGLSIKN*
Ga0136644_1001229510	3300010334	Freshwater Lake	MSYKISDAVAMRFIQIFQEAIMLGVDGADLMRQVRLVVDPEDDSTVTLDPQYVSLVEEMHRQFLDRAEALKKEQDSQRLILEN*
Ga0136644_100551042	3300010334	Freshwater Lake	MSYKISDAVAMRFIQIFQEAVLLGVDGADLMRQVRLTVDPTDSTTVTLDPQYEAQVADMHRKYLEEAEKLQAAKSQNDSQTKLIFES*
Ga0129333_1000002326	3300010354	Freshwater To Marine Saline Gradient	MEVYMSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDESNSDTLTLDPSYQGQVADMHKKYLEDAERLQSQQKKNSDNVFTLNL*
Ga0129333_100015182	3300010354	Freshwater To Marine Saline Gradient	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSTDVFSS*
Ga0129333_1000561014	3300010354	Freshwater To Marine Saline Gradient	MSYKISDSVAMRFIQIFQEAVLMGVDGADLMRQVRLTVDSTNEDTLTLCPEYERMVEAQHKKYLEDAEKLKSQSGDSGLTTKMIFES*
Ga0129333_100639795	3300010354	Freshwater To Marine Saline Gradient	MSYKISDSVAMRMIQIFQEALLLGVDGADLMRQVRLVVDSENPDTLTLDPQYVDQVNQMHQKYLAQAESLKASETNQLKIIFDNN*
Ga0129333_102607883	3300010354	Freshwater To Marine Saline Gradient	MIDTKVRINCKSIERYIMSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLTLDSTNEGTLTLCPEYEQMVEAQHKKYLEDAEKLKTQTEVSADLPKLIFEN*
Ga0151516_1107310	3300011116	Freshwater	MSYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVVDEKDADTVTLCPTYEKQVVEMHKKYLDDVEKLTAVSESSRPGLSIKN*
Ga0119951_10542621	3300012000	Freshwater	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLTVDSEDAGVLTLDPEYEVQVANTHKKYLEDAEKLKSERSSKEEVKLVFEN*
Ga0153805_10028992	3300012013	Surface Ice	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLQDAEKLKAQTESTSELPKLIFEN*
Ga0164295_115989051	3300013014	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDEANPDTVMLCPQYENQVLEMHKKYLEDAERLRARSES
(restricted) Ga0172367_100784735	3300013126	Freshwater	MNYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVVDESSPDTMTLDPQYEEMVESQHKKYLEDAEKLKSQTHESSVPKLIFEN*
(restricted) Ga0172367_101106521	3300013126	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTPDTVTLCPDYVKQVTEMHQKYLDDAEKLKVVSESSRPGFVLES*
(restricted) Ga0172367_102381302	3300013126	Freshwater	MSYKISDSVAMRMIQIFQEALLLGVDGADLMRQVRLVVDFSNPDVMTLDPQYESQVSEMHQKYLDEAEKLKQSSSGEVLVFES*
(restricted) Ga0172373_100025397	3300013131	Freshwater	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLTVDSENPDTMTLDPQYKDQVGQMHQKYLEEAEALRTAEQNKQLGLNFS*
(restricted) Ga0172373_100077763	3300013131	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLIIDESQPDTVTLCPEYEKQVIEMHKKYLEDAEMLQSSKTEPIN*
(restricted) Ga0172373_101188002	3300013131	Freshwater	MSYKISDAVSIRMIQIFQEALILGVDGADLMRQVRLVVDENNTDTLTLDPTYEKQVADMHAKYLAEAESLKENSLKEQDQFKLVFEN*
(restricted) Ga0172373_102343011	3300013131	Freshwater	MSYKISDSVAMRMIQIFQEAVLLGLDGADLMRQVRLVVDSSQPDTVTLDPTYEAQVAEMHKKYLSDAEKLATSTVKLPNVIS*
(restricted) Ga0172372_101249732	3300013132	Freshwater	MSYKMSDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDAEHPDTVTLDPAYEQQVQSMHKKYLEEAETLKSKLNDRSGDSRLIFES*
Ga0177922_112322829	3300013372	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTSELPKLIFEN*
Ga0119952_11085682	3300014050	Freshwater	MNYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDTTSPDTLTLDPGYEAQVAEMHRKYLEDAQRLKETQDRQEVLVFER*
(restricted) Ga0172376_102705141	3300014720	Freshwater	MSYKISDSVAMRMIQIFQEALLLGVDGADLMRQVRLVVDFSNPDVMTLDPQYESQVSEMHQKYLDEAEKLKQSSSGEVLVFE
Ga0181343_10049272	3300017766	Freshwater Lake	MSYKISDAVAMRMIQIFQEALLLGLDGADLMRQVRLVVDDTDPGTVTLDPQYEKQVIEMHEKYLQDAEKLKSQSTGTEGPKLIFEN
Ga0181343_12118152	3300017766	Freshwater Lake	SYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEKDTDTVTLCPTYEKQVVEMHKKYLDDVEKLTAVSESSRPGLSIKN
Ga0181357_11412013	3300017777	Freshwater Lake	LMYGFIMSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDEANPDTVVLCPQYENQVLEMHKKYLEDAERLKARSESITVETPRLILEN
Ga0181348_11737892	3300017784	Freshwater Lake	MYGFIMSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDEANPDTVVLCPQYENQVLEMHKKYLEDAERLKTRSESITVETPRLILEN
Ga0169931_103581762	3300017788	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTPDTVTLCPDYVKQVTEMHQKYLDDAEKLKVVSESSRPGFVLES
Ga0169931_107810402	3300017788	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGVDGADLMRQVRLTVDSTNEDTLTLCPEYERMVEAQHKKYLEDAEKLKSESSDSGSTSKVIFEN
Ga0194113_100681596	3300020074	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGVDGADLMRQVRLTVDSTNEDTLSLCPAYERMVEAQHKKYLEDAEKLKTQSNDFNSTSKVIFEN
Ga0194113_101116372	3300020074	Freshwater Lake	MSYKISDAVSMRFIQIFQEAVLMGLDGADLMRQVRLVVSDTEADTVTLCPEYEKQVIEMHKKYLEEAEQLKSQSQDASITPKILFEN
Ga0194113_101237192	3300020074	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTPNTMTLCPDYVKQVAEMHQKYLDDAEKLKVVSESSRLVSS
Ga0194113_103155932	3300020074	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDASTPDTVTLCPDYVKQVAEMHQKYLDDAEKLKTQN
Ga0194111_101864371	3300020083	Freshwater Lake	FQEAVLMGLDGADLMRQVRLVVSDTEADTVTLCPEYEKQVIEMHKKYLEEAEQLKSQSQDASITPKILFEN
Ga0194112_103712533	3300020109	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDAEPDTMTLSPDYEKQVVEMHKKYLEDAEKLSVVSESSRPG
Ga0211729_111333305	3300020172	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN
Ga0211729_112592136	3300020172	Freshwater	MFRYLDTSRRIEIQSFFMSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVDEMHRKYLEEAELLKTSSTEVFSS
Ga0194134_100814343	3300020179	Freshwater Lake	MSYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVVDESSPDTMTLDPQYEEMVESQHKKYLEDAEKLKSQTHESSVPKLIFEN
Ga0194115_100102422	3300020183	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDAEPDTMTLSPDYEKQVVEMHKKYLEDAEKLSVVSESSRPGLIFEN
Ga0194115_100392754	3300020183	Freshwater Lake	MSYKISDAVAMRFIQIFQEAVLMGIDGADLMRQVRLVIDESAQDTLTLDPLYEKLVEDQHKKYLEDAELLKSQVELTTPKIIFEN
Ga0194115_100925012	3300020183	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDESTPDTVALCPDYVKQVAEMHQKYLDDAEKLKTQN
Ga0194115_101318242	3300020183	Freshwater Lake	MSYKISDSVALRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTSDTMTLCPDYVKQVAEMHQKYLDDAEKLKIVSESSRPDFTENN
Ga0194115_101541042	3300020183	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDDATPDTVTLCPAYEKQVAEMHKKYLEDAEKLASQSNLKN
Ga0194131_1000787211	3300020193	Freshwater Lake	MFFMSYKISDSVALRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTSDTMTLCPDYVKQVAEMHQKYLDDAEKLKTVSESSRPDFTENN
Ga0194131_1001437610	3300020193	Freshwater Lake	MNYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVADESSPDTMTLDPQYEEMVESQHKKYLEDAEKLKSQTHESSVSKLIFEN
Ga0194124_103834632	3300020196	Freshwater Lake	ISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDESSPDTVTLCPKYEKQVFEMHKTYLEDAEKLKSQTSDVIQTPKVIFEN
Ga0194128_102327102	3300020197	Freshwater Lake	MSYKISDSVALRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTSDTMTLCPDYVKQVAEMHQKYLDDAEKLKTVSESSRPDFTENN
Ga0194120_104885412	3300020198	Freshwater Lake	KISDSVALRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTSDTMTLCPDYVKQVAEMHQKYLDDAEKLKTVSESSRPDFTENN
Ga0194121_101613742	3300020200	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDESSPDTVTLCPKYEKQVFEMHKTYLEDAEKLKSQTSDVIQTPKVIFEN
Ga0194116_100694061	3300020204	Freshwater Lake	IMSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDASTPDTVTLCPDYVKQVAEMHQKYLDDAEKLKTQN
Ga0211731_117343702	3300020205	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPGTVTLCPQYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN
Ga0194132_100108588	3300020214	Freshwater Lake	MNYKISDSVAMRFVQIFQEAVLLGVDGADLMRQVRLVVDESSPDTMTLDPQYEEMVESQHKKYLEDAEKLKSQTHESSVPKLIFEN
Ga0208697_10353462	3300020507	Freshwater	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLVVDESNPDTVTLDPKYELQVSEMHKKYLAEAESLKDKKDSQGVLVFE
Ga0194133_100013602	3300021091	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLTVDPSDNNSLTLCPEYEKMVESQHKKYLEDAEKLKTQTDQAADFPKLVFEN
Ga0194133_103450781	3300021091	Freshwater Lake	DSVALRFIQIFQEAVLLGVDGADLMRQVRLVVDDSTSDTMTLCPDYVKQVAEMHQKYLDDAEKLKIVSESSRPDFTENN
Ga0214917_10000016142	3300022752	Freshwater	MNYKISDSVAMRFIQIFQEAILMGVDGADLMRQVRLTVDESTPDTLTLHPEYEKMVEAQHKKYLEDAEKLKSQSETLPNVPKLIFEN
Ga0214917_1000283123	3300022752	Freshwater	MNYKISDSVAMRFIQIFQEAVLMGVDGADLMRQVRLTVDESSPDTLTLHPEYEKMVEAQHKKYLEDAEKLKSQSETLTDVPKLIFEN
Ga0214917_100685881	3300022752	Freshwater	MNGDKIMSYKISDKVAMRMIQIFQEALLLGVDGADLMRQVRLSQDESDSGTLTLDPQYERQVAEMHRKYLEEAESLKSKSNDDVFKINFSG
Ga0214917_100849282	3300022752	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVIDESAPDTVTLCPEYQKQVAEMHKKYLEDAEKLQASNTVLNLNG
Ga0214917_101177161	3300022752	Freshwater	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLTVDSEDAGVLTLDPEYEVQVANTHKKYLEDAEKLKSERSSKEEVKLVFEN
Ga0214917_101597013	3300022752	Freshwater	MNYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDTTSPDTLTLDPGYEAQVAEMHRKYLEDAQRLKETQDRQEVLVFER
Ga0214917_102098652	3300022752	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN
Ga0214917_102818492	3300022752	Freshwater	MSYKISDSVAMRFIQIFQEAILLGIDGADLMRQVRLVTDAQDPGTMTLCPEYEKQVVEAHKKYLEEAEKLKSQTADLSSTPKILFEN
Ga0214917_104011981	3300022752	Freshwater	MSYKISDSVAMRMIQIFQEALLLGVDGADLMRQVRLVVDSENLDTLTLDPQYVDQVNQMHQKYLAQAESLKASETNQLKIIFDNN
Ga0214921_100165326	3300023174	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEKDTDTVTLCPTYEKQVVEMHKKYLDDVEKLTAVSESSRPGLSIKN
Ga0214921_100739485	3300023174	Freshwater	MSYKISDSVAMRMIQIFQEALLLGLDGADLMRQVRLVVDSSTPDVLTLDPQYESQVSEMHQKYLDEAEKLAQASSGEVLVFER
Ga0214921_100763325	3300023174	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDETSADTITLCPQYEAQVIEMHKKYLQDAEKLKTQTESTSELPKLIFEN
Ga0214921_101624541	3300023174	Freshwater	FIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN
Ga0214923_100124418	3300023179	Freshwater	MSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDENNNDTLTLDPAYQGQVAEMHRKYVEDAERLQSQQKQSTDNVFRLTL
Ga0214923_100674893	3300023179	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLLFEN
Ga0214919_1000901218	3300023184	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDETSTDTVTLCPQYETQVTEMHQKYLQDAERLKNKTAESLPELPKLVFES
Ga0214919_100535945	3300023184	Freshwater	MSYKISDAVAMRFIQIFQEAVLLGVDGADLMRQVRLTVDPTDSTTVTLDSQYEAQVADMHRKYLEEAEKLQAAKSQNDSQTKLIFES
Ga0214919_103087632	3300023184	Freshwater	MSYKISDLVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDSNDETTVTLDPQYELQVADMHRKYLEDAERLKSQASVTESKLIFES
Ga0247724_100002619	3300024239	Deep Subsurface Sediment	MSYKISDAVAMRMIQIFQEALLLGVDGADLMRQVRLVVSPEDAGVLTLDPQYEKQVFDMHQKYLEEAEALKSKLDDQSIVKLTFDN
Ga0255205_10418931	3300024276	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSTDVFSS
Ga0255215_10346021	3300024278	Freshwater	KISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSSTDVFSS
Ga0255209_10439762	3300024280	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSSTDVFSS
Ga0244777_104237252	3300024343	Estuarine	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLDDAEKLKTQTESTELPKLIFEN
Ga0244775_112072362	3300024346	Estuarine	LNKIFVLMYGFIMSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVIDEANPDTVVLCPQYENQVLEMHKKYLEDAERLRTRSESITT
Ga0255186_10113141	3300024512	Freshwater	SVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSTDVFSS
Ga0255237_10580471	3300024564	Freshwater	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPKYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN
Ga0208019_11209691	3300025687	Aqueous	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDETTPDTVTLCPEYEKQVTEMHKKYLEEAEKLSTVSESSRPGLV
Ga0255198_10689241	3300027160	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKT
Ga0208974_10282003	3300027608	Freshwater Lentic	MSYKISDSVAMRFIQIFQEAVLLGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVIEMHKKYLQDAEKLKAQTESTSELPKLIFEN
Ga0208133_11271281	3300027631	Estuarine	LNKIFVLMYGFIMSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDEVNPDTVMLCPQYENQVLEMHKKYLEDAERLRTRSESITTE
Ga0209087_12954021	3300027734	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPQYEAQVLEMHKKYLDDAEKLKTQTESTELPKLLFEN
Ga0209189_10455961	3300027747	Freshwater Lake	IQIFQEAIMLGVDGADLMRQVRLVVDPEDDSTVTLDPQYVSLVEEMHRQFLDRAEALKKEQDSQRLILEN
Ga0209189_10931193	3300027747	Freshwater Lake	MSYKISDAVAMRFIQIFQEAVLLGVDGADLMRQVRLTVDPTDSTTVTLDPQYEAQVADMHRKYLEEAEKLQAAKSQNDSQTKLIFES
Ga0209596_10001564	3300027754	Freshwater Lake	MSYKISDSVAMRFIQIFQEAVLMGIDGADLMRQVRLVVDETNPDTVVLCPQYENQVLEMHKKYLEDAERLRARSESITTETPRLILEN
Ga0209298_1000342813	3300027973	Freshwater Lake	MSYKISDSVAMRMIQIFQEALLLGLDGADLMRQVRLVVDSSAPDVLTLDPQYESQVSEMHQKYLDEAEKLAQASSREVLVFER
Ga0255216_10470382	3300028071	Freshwater	MSYKISDSVAMRFIQIFQEAVLLGIDGADLMRQVRLVVDETTPDTVTLCPAYEKQVAEMHQKYLEEAELLKTSSTDVF
Ga0256305_11462941	3300028108	Freshwater	DSVAMRFIQIFQEAVLMGLDGADLMRQVRLVVDEASPDTVTLCPKYEAQVLEMHKKYLDDAEKLKTQTESTELPKLIFEN
Ga0310130_0000207_14222_14479	3300034073	Fracking Water	MSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDESSPDTVTLDPQYEKQVADMHQKYLSDAEDLKAKTQQTTSQLVFGN
Ga0310130_0000361_10093_10347	3300034073	Fracking Water	MSYKISDSVAMRFIQIFQEAVLLGVDGADLMRQVRLVVDDKEPDTVTLCPQYEKQVVEMHKKYLEDAEKLALSGNSGTNSGFNH
Ga0310130_0117609_309_566	3300034073	Fracking Water	MSYKISDSIAMRMIQIFQEAVLLGVDGADLMRQVRLVVNESQPDTLTLDPQYEKQVAEMHEKYLKESEHLKEKTESRSQKLLFES
Ga0335027_0000012_53094_53351	3300034101	Freshwater	MSYKISDTVAMRMIQIFQEALLLGVDGADLMRQVRLVVDSNNPDTVTLDPEYEKQVAEMHKKYLDEAEALKAKQAGTSVPLIFEN
Ga0335027_0094977_22_282	3300034101	Freshwater	MSYKISDAVAMRMIQIFQEALILGVDGADLMRQVRLVVDESSTDTLTLDPQYEAQVTEMHKKYLEDAEMLRARQQSENQTNLVFEN